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Various Multicharged Anions of Ginsenosides in Negative Electrospray Ionization with QTOF High-Resolution Mass Spectrometry
Zhao, Nan1,2; Cheng, Mengchun1; Huang, Shuai1; Liu, Dan1; Zhao, Qiang1,3; Bai, Yunpeng1; Zhang, Xiaozhe1
Corresponding AuthorZhang, Xiaozhe(zhangxz@dicp.ac.cn)
KeywordElectrospray ionization Ginsenoside Multicharged anion Multimer High-resolution mass spectrometry
Source PublicationJOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
2019-03-01
ISSN1044-0305
DOI10.1007/s13361-018-2089-5
Volume30Issue:3Pages:403-418
Funding ProjectMinistry of Science and Technology of the People's Republic of China[2015DFG42460] ; Bureau of International Co-operation Chinese Academy of Sciences[121421KYSB20160006] ; National Key R&D Program of China[2016YFC1306603] ; National Science Foundation[21475128] ; Chinese Academy of Science
Funding OrganizationMinistry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical ; Chemistry, Physical ; Spectroscopy
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Spectroscopy
WOS KeywordPANAX-QUINQUEFOLIUS ; AMERICAN GINSENG ; ASIAN GINSENG ; DIFFERENTIATION ; DISCRIMINATION ; LEAVES ; RH2 ; RG1
AbstractWhen characterizing components from ginseng, we found a vast number of multicharged anions presented in the liquid chromatography-mass spectrometry (LC-MS) chromatograms. The source of these anions is unclear yet, while ginsenosides, the major components of ginseng, are the main suspected type of molecules because of their sugar moiety. Our investigation using 14 pure ginsenosides affirmed that the multicharged anions were formed by ginsenosides rather than other types of ingredients in ginseng. Various anions could be observed for each ginsenoside. These anions contain ions ([M-2H](2-), [M+Adduct](2-)), as well as those formed by polymerization of at least two ginsenosides, such as [nM-2H](2-), [nM-H+Adduct](2-), and [nM-3H](3-). The presence of so different types of ions from a ginsenoside explains the reason for the large number of anions in the LC-MS analysis of ginseng. We further found that formation of [nM-2H](2-) ions was influenced by the number of sugar chains: ginsenosides containing two sugar chains produced all [nM-2H](2-) ion types, whereas ginsenosides containing one sugar chain did not produce [2M-2H](2-). Thus, [2M-2H](2-) and [3M-2H](2-) can be utilized to rapidly identify monodesmosidic and/or bidesmosidic ginsenosides as joint diagnostic anions. The position of the glycosyl radical might be the key factor affecting the formation of multicharged multimer ions from monodesmosidic ginsenosides. Consequently, three groups of ginsenoside isomers were differentiated by characteristic [nM-2H](2-) anions. Using concentration-dependent characteristics and collision-induced dissociation (CID), we confirmed that [nM-2H](2-) ions are non-covalently bound multimers whose aggregation has marked distinction between monodesmosidic and bidesmosidic ginsenosides, accounting for the differentiated formation of [nM-2H](2-) between them.
Language英语
Funding OrganizationMinistry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; Bureau of International Co-operation Chinese Academy of Sciences ; Bureau of International Co-operation Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation ; National Science Foundation ; Chinese Academy of Science ; Chinese Academy of Science
WOS IDWOS:000461325300002
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165841
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Xiaozhe
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Zhongshan Rd 457, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China
3.Shenyang Pharmaceut Univ, Minist Educ, Key Lab Struct Based Drug Design & Discovery, Shenyang, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Nan,Cheng, Mengchun,Huang, Shuai,et al. Various Multicharged Anions of Ginsenosides in Negative Electrospray Ionization with QTOF High-Resolution Mass Spectrometry[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2019,30(3):403-418.
APA Zhao, Nan.,Cheng, Mengchun.,Huang, Shuai.,Liu, Dan.,Zhao, Qiang.,...&Zhang, Xiaozhe.(2019).Various Multicharged Anions of Ginsenosides in Negative Electrospray Ionization with QTOF High-Resolution Mass Spectrometry.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,30(3),403-418.
MLA Zhao, Nan,et al."Various Multicharged Anions of Ginsenosides in Negative Electrospray Ionization with QTOF High-Resolution Mass Spectrometry".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 30.3(2019):403-418.
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