DICP OpenIR
Surface modification with highly-homogeneous porous silica layer for enzyme immobilization in capillary enzyme microreactors
Liu, Xin1; Zhu, Xudong2; Camara, Mohamed Amara1; Qu, Qishu3; Shan, Yichu2; Yang, Li1
Corresponding AuthorShan, Yichu(shanyichu@dicp.ac.cn) ; Yang, Li(yang1330@nenu.edu.cn)
KeywordSurface modification Porous layer open tubular Immobilized enzyme reactor Microchannel
Source PublicationTALANTA
2019-05-15
ISSN0039-9140
DOI10.1016/j.talanta.2019.01.080
Volume197Pages:539-547
Funding ProjectNational Natural Science Foundation of China[21775017] ; National Natural Science Foundation of China[21475019] ; Natural Science Foundation of Jilin Province, China[20180101174JC] ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University)
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University)
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMEDIATED MICROANALYSIS ; REACTORS ; ELECTROPHORESIS ; FLOW ; INHIBITORS ; COLUMNS ; ACETYLCHOLINESTERASE ; PROTEIN ; CHIP
AbstractImmobilized enzyme micro-reactors (IMERs) are of vital importance in developing miniaturized bioanalytical systems and have promising applications in various biomanufacturing. An inherent limitation in designing IMERs is the one-dimensional cylindrical geometry of micro-channels that offers limited exposed surface area for molecular reorganization and enzyme immobilization. In this study, we report a robust capillary-IMER based on a three dimensional porous layer open tubular (3D-PLOT) column which is prepared by an easy-to-control surface modification strategy via single-step in situ biphasic reaction. The 3D-PLOT column with highly uniform porous geometry and narrow distribution of porosity can greatly enhance the surface-area-to-volume ratio of the micro-channels, showing the beneficial effects for enzyme immobilization to enhance reaction efficiency and shorten analysis time. Taking trypsin as a model enzyme, enzymatic activities of immobilized enzyme are analyzed. We compare enzyme assays using the proposed 3D-PLOT-IMER with those using normal capillary-IEMR without surface modification as well as free trypsin. The 3D-PLOT-IMER exhibits excellent stability and inter/intra-day reproducibility, and these characteristics imply the reliability of the proposed IMERs for accurate enzyme assay. The feasibility of the proposed method for potential application in biological analysis is demonstrated by coupling the 3D-PLOT-IMER with a nano-LC-MS/MS system for online digestion of standard proteins, cell extraction and living Hela cells. Our study show that the surface modification with the proposed 3D-porous layer is a simple and efficient approach for enzyme immobilization, and could be widely suitable for different kinds of IMERs.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province, China ; Natural Science Foundation of Jilin Province, China ; Jilin Provincial Department of Education ; Jilin Provincial Department of Education ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University) ; Jilin Provincial Key Laboratory of Micro Nano Functional Materials (Northeast Normal University)
WOS IDWOS:000460710200071
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165853
Collection中国科学院大连化学物理研究所
Corresponding AuthorShan, Yichu; Yang, Li
Affiliation1.Northeast Normal Univ, Dept Chem, Key Lab Nanobiosensing & Nanobioanal Univ Jilin P, 5268 Renmin St, Changchun 130024, Jilin, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Anhui Jianzhu Univ, Sch Mat & Chem Engn, Key Lab Funct Mol Design & Interface Proc, Hefei 230601, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xin,Zhu, Xudong,Camara, Mohamed Amara,et al. Surface modification with highly-homogeneous porous silica layer for enzyme immobilization in capillary enzyme microreactors[J]. TALANTA,2019,197:539-547.
APA Liu, Xin,Zhu, Xudong,Camara, Mohamed Amara,Qu, Qishu,Shan, Yichu,&Yang, Li.(2019).Surface modification with highly-homogeneous porous silica layer for enzyme immobilization in capillary enzyme microreactors.TALANTA,197,539-547.
MLA Liu, Xin,et al."Surface modification with highly-homogeneous porous silica layer for enzyme immobilization in capillary enzyme microreactors".TALANTA 197(2019):539-547.
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