DICP OpenIR
The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material
Luo, Jian1; Li, Qian1; Sun, Xitong2; Tian, Jing1; Fei, Xu1; Shi, Feng1; Zhang, Nan1; Liu, Xiangli1
Corresponding AuthorLi, Qian(liqian19820903@163.com) ; Sun, Xitong(sunxt@dicp.ac.cn)
Source PublicationRSC ADVANCES
2019
ISSN2046-2069
DOI10.1039/c9ra00075e
Volume9Issue:8Pages:4514-4520
Funding ProjectNational Natural Science Foundation of China[31601411] ; National Natural Science Foundation of China[21804129]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordCATALYTIC-ACTIVITY ; MESOPOROUS SILICA ; NANOPARTICLES
AbstractSilica material has high specific surface area and excellent chemical stability, which make it useful for enzyme immobilization. In this work, naringinase was immobilized from fermentation broth of Aspergillus niger FFCC uv-11 by silica materials with different pore diameters of 2 nm (MCM-41), 7.7 nm (SBA-15) and 80 nm (silica gel). It was shown that SBA-15 had the highest naringinase activity, and this was chosen as a suitable carrier material for naringinase immobilization. First, SBA-15 was modified by glutaraldehyde at a concentration of 7% at 25 degrees C for 2 h, and it was then used for the immobilization of naringinase. At pH 3.5, the immobilized naringinase activity reached 467.62 U g(-1) at 40 degrees C for 4 h when the initial naringinase activity was 89.04 U mL(-1). Furthermore, at the optimal reaction temperature of 45 degrees C and pH of 4.5, the binding efficiency, activity recovery rate and specific activity of the immobilized naringinase were 63.66%, 87.64% and 517.43 U g(-1), respectively. Compared with free naringinase, in naringin hydrolysis, the immobilized naringinase performed over a wide pH application range and had good thermal stability. Even more important, the immobilized naringinase retained 61.81% of the residual naringinase activity after eight consecutive cycles, and kept 80.95% of the residual naringinase activity after one month of storage. This study provides an ideal carrier material and some basic data for naringinase immobilization technology, which will greatly promote the application of naringinase in industrial fruit juice processing.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000459896700047
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165869
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Qian; Sun, Xitong
Affiliation1.Dalian Polytech Univ, Dept Bioengn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Luo, Jian,Li, Qian,Sun, Xitong,et al. The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material[J]. RSC ADVANCES,2019,9(8):4514-4520.
APA Luo, Jian.,Li, Qian.,Sun, Xitong.,Tian, Jing.,Fei, Xu.,...&Liu, Xiangli.(2019).The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material.RSC ADVANCES,9(8),4514-4520.
MLA Luo, Jian,et al."The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material".RSC ADVANCES 9.8(2019):4514-4520.
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