DICP OpenIR
Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells
Liu, Lukuan1,2,3; Yang, Kaiguang1; Gao, Hang1,2; Li, Xiao1; Chen, Yuanbo1,2; Zhang, Lihua1; Peng, Xiaojun; Zhang, Yukui1,3
Corresponding AuthorZhang, Lihua(lihuazhang@dicp.ac.cn)
Source PublicationANALYTICAL CHEMISTRY
2019-02-19
ISSN0003-2700
DOI10.1021/acs.analchem.8b05259
Volume91Issue:4Pages:2591-2594
Funding ProjectNational Key Research and Development Program of China[2017YFA0505003] ; National Key Research and Development Program of China[2016YFA0501401] ; National Natural Science Foundation[21575143] ; National Natural Science Foundation[91543201] ; National Natural Science Foundation[21725506] ; CAS Key Project in Frontier Science[QYZDY-SSW-SLH017]
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCANCER-CELLS ; RELEASE ; RECOGNITION ; BINDING
AbstractIsolation of circulating tumor cells (CTCs) from blood holds great potential to diagnose cancers and discover therapeutic targets. Herein, we reported a novel kind of artificial antibody, the cell-imprinted hydrogel with site-directed modification of aptamers (APT-CIH) to achieve the specific capture of CTCs. Cell-imprinted sites not only could be used to recognize target cells but also could be used as efficient scaffolds for assembling aptamers to enhance the capture efficiency and selectivity. Due to the synergistic effect of conformation recognition and multivalent interaction between the aptamers and target cells, APT-CIH showed high capture efficiency and selectivity to SMMC-7721 cells. In the coexistence of the 1000 times leukemia Jurkat cells, the enrichment factor of APT-CIH could reach as high as 21.6 +/- 3.1 toward target cells, while that relied only on cell imprinting or aptamer affinity was 8.1 +/- 5.0 or 10.1 +/- 1.3, respectively. Furthermore, the capture efficiency could reach 58.2% +/- 10.9% with 1000 SMMC-7721 cells spiked in 1 mL of blood. Moreover, 92% of the captured cells could be released, beneficial to carry out further biological and clinical study of CTCs. These results demonstrated that APT-CIH might have great potential in CTCs analysis.
Language英语
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science
WOS IDWOS:000459642400005
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165977
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lukuan,Yang, Kaiguang,Gao, Hang,et al. Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells[J]. ANALYTICAL CHEMISTRY,2019,91(4):2591-2594.
APA Liu, Lukuan.,Yang, Kaiguang.,Gao, Hang.,Li, Xiao.,Chen, Yuanbo.,...&Zhang, Yukui.(2019).Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells.ANALYTICAL CHEMISTRY,91(4),2591-2594.
MLA Liu, Lukuan,et al."Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells".ANALYTICAL CHEMISTRY 91.4(2019):2591-2594.
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