DICP OpenIR
Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization
Bao, Ruiqi1; Gao, Ning2; Lv, Jing1; Ji, Chaofan1; Liang, Huipeng1; Li, Shengjie1; Yu, Chenxu3; Wang, Zhenyu1; Lin, Xinping1
Corresponding AuthorWang, Zhenyu(wangzy0506@outlook.com) ; Lin, Xinping(yingchaer@163.com)
KeywordRhodosporidium toruloides torularhodin Agrobacterium tumefaciens-mediated transformation response surface methodology selective production
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
2019-01-30
ISSN0021-8561
DOI10.1021/acs.jafc.8b04667
Volume67Issue:4Pages:1156-1164
Funding ProjectNational Natural Science Foundation of China[31501464] ; High Level Talents Innovation and Entrepreneurship Project of Dalian[2017RQ045] ; Natural Science Foundation of Liaoning Province[20170540057] ; Educational Commission of Liaoning Province[2016J018]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS KeywordYEAST RHODOTORULA-GLUTINIS ; CAROTENOID PRODUCTION ; BETA-CAROTENE ; LIPID PRODUCTION ; C/N RATIO ; GLYCEROL ; GROWTH ; MUTANT ; TEMPERATURE ; PROFILES
AbstractNine transformants of Rhodosporidium toruloides with significant changes in the carotenoid profile were obtained by Agrobacterium tumefaciens-mediated transformation, including a white, three red, and four yellow mutants. A red mutant Al-15-BRQ that showed a high torularhodin production was selected for culture condition optimization. Results indicated that the torularhodin yield was boosted with glucose as the carbon source, at a carbon/nitrogen ratio of 22, a loading volume of 75 mL, and 28 degrees C. The torularhodin yield of 21.3 mg/L consisting of 94.4% total carotenoids was obtained by Box-Behnken design experiments. The torularhodin yield was 17.0-fold higher than that of the wild type, with time shortened from 9 to 3 days. This study reports an effective strategy for improving torularhodin production and provides a candidate R toruloides strain for highly selective production of torularhodin.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province
WOS IDWOS:000457815800014
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166151
Collection中国科学院大连化学物理研究所
Corresponding AuthorWang, Zhenyu; Lin, Xinping
Affiliation1.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, 1 Qinggongyuan, Dalian 116034, Liaoning, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
3.Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA
Recommended Citation
GB/T 7714
Bao, Ruiqi,Gao, Ning,Lv, Jing,et al. Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(4):1156-1164.
APA Bao, Ruiqi.,Gao, Ning.,Lv, Jing.,Ji, Chaofan.,Liang, Huipeng.,...&Lin, Xinping.(2019).Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(4),1156-1164.
MLA Bao, Ruiqi,et al."Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.4(2019):1156-1164.
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