DICP OpenIR
Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides
Ji, Yongsheng1; Lv, Ruihong1; Song, Shuhui1; Huang, Junfeng2; Zhang, Luwei2; Huang, Guang2; Li, Jinan2; Ou, Junjie2
Corresponding AuthorJi, Yongsheng(jiysh@hactcm.edu.cn) ; Ou, Junjie(junjieou@dicp.ac.cn)
KeywordZwitterionic polymer Magnetic composites Hydrophilic interaction chromatography Glycopeptides enrichment Distillation-precipitation polymerization
Source PublicationANALYTICA CHIMICA ACTA
2019-04-11
ISSN0003-2670
DOI10.1016/j.aca.2018.12.003
Volume1053Pages:43-53
Funding ProjectNational Natural Sciences Foundation for young scientists of China[21505034] ; science and technology innovation talent support program of Henan University of Chinese Medicine[2016XCXRC02] ; training plan for young key teachers in universities of Henan province[2017GGJS079] ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine[2014KYYWF-QN15]
Funding OrganizationNational Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; National Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; National Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; National Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHIGH-EFFICIENT ENRICHMENT ; METAL-ORGANIC FRAMEWORK ; N-LINKED GLYCOPEPTIDES ; SELECTIVE ENRICHMENT ; STATIONARY PHASES ; CLICK SYNTHESIS ; NANOPARTICLES ; NANOCOMPOSITES ; MICROSPHERES ; SEPARATION
AbstractHydrophilic interaction chromatography (HILIC) utilizing zwitterion-modified material as solid phase has attracted extensive attention for selective enrichment of glycopeptides. However, a tedious synthesis and low specificity for glycopeptides have restricted its application. Herein, a facile and effective approach was developed to synthesize a zwitterionic (ZIC) polymer-coated magnetic composites (denoted as Fe3O4@PMSA) with a zwitterion ((2-(methacryloyloxy) ethyl)-dimethyl-(3-sulfopropyl) ammonium hydroxide, MSA) via one-step distillation-precipitation polymerization (DPP). The well-designed composites presented clearly ZIC-polymer shell and superior hydrophilicity (water contact angle 30.2), and the performance for selective enrichment of glycopeptides were investigated with standard and real samples, respectively. Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N >= 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/N >= 20). Besides, the detection limit as low as 0.67 fmol for IgG (S/N >= 10) and satisfied recovery yield more than 74% were achieved by the proposed sorbents. Finally, the Fe3O4@PMSA was applied for enriching N-linked glycopeptides from human serum, and 348 unique N-glycosylation sites and 419 glycopeptides from 158 glycoproteins were strictly identified from 1 mL human serum. The results demonstrated that the proposed Fe3O4@PMSA showed a promising potential in glycoproteomics analysis of real biological samples. (C) 2018 Elsevier B.V. All rights reserved.
Language英语
Funding OrganizationNational Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; National Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; National Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; National Natural Sciences Foundation for young scientists of China ; National Natural Sciences Foundation for young scientists of China ; science and technology innovation talent support program of Henan University of Chinese Medicine ; science and technology innovation talent support program of Henan University of Chinese Medicine ; training plan for young key teachers in universities of Henan province ; training plan for young key teachers in universities of Henan province ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine ; Special support program for basic scientific research fund of Henan Provincial University in Henan University of Chinese Medicine
WOS IDWOS:000457201600004
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166167
Collection中国科学院大连化学物理研究所
Corresponding AuthorJi, Yongsheng; Ou, Junjie
Affiliation1.Henan Univ Chinese Med, Coll Pharm, Zhengzhou 450046, Henan, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Ji, Yongsheng,Lv, Ruihong,Song, Shuhui,et al. Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides[J]. ANALYTICA CHIMICA ACTA,2019,1053:43-53.
APA Ji, Yongsheng.,Lv, Ruihong.,Song, Shuhui.,Huang, Junfeng.,Zhang, Luwei.,...&Ou, Junjie.(2019).Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides.ANALYTICA CHIMICA ACTA,1053,43-53.
MLA Ji, Yongsheng,et al."Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides".ANALYTICA CHIMICA ACTA 1053(2019):43-53.
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