DICP OpenIR
Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition
Li, Hua1; Qin, Qian2,4; Shi, Xianzhe2; He, Jun3; Xu, Guowang2
Corresponding AuthorShi, Xianzhe(shixianzhe@dicp.ac.cn)
KeywordModified metabolites All ion fragmentation Neutral loss Nontargeted analysis Metabolomics
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2019-01-04
ISSN0021-9673
DOI10.1016/j.chroma.2018.11.014
Volume1583Pages:80-87
Funding ProjectNational Natural Science Foundation of China[21575142] ; National Natural Science Foundation of China[21505132] ; National Natural Science Foundation of China[21705147] ; National Natural Science Foundation of China[21435006]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMODIFICATION-SPECIFIC METABOLOMICS ; MODIFIED NUCLEOSIDES ; IDENTIFICATION ; PROTEOMICS
AbstractModified metabolites play important roles in diagnostic monitoring, oxidative response and physiological regulation. Comprehensive analytical methods are greatly needed for improving the coverage of modified metabolites and studying their physiological function. Here, a novel nontargeted profiling method for mapping modified metabolites was developed by liquid chromatography-high resolution mass spectrometry with full scan/all ion fragmentation/neutral loss (FS/AIF/NL) data acquisition. Modified metabolites were unbiasedly defined with less false positive results by mixed standards verification. Thirteen types of modified metabolites in urine were analyzed at the same time, and 198 of 307 modified metabolites at positive mode and 166 of 366 modified metabolites at negative mode were putatively identified. The modified metabolites profiling method was applied for investigating the metabolic differences between the patients with breast cancer and health controls. As a result, many modified metabolites with glu-curonidation, ribosylation and indole acetylation modification were significantly up-regulated in breast cancer and four modified metabolites including 7-methylguanosine, N-4-acetylcytidine, dihyroxy-1H-indole glucuronide I and indole-3-acetic acid-O-glucuronide were identified as potential biomarkers for the diagnose of breast cancer. (C) 2018 Elsevier B.V. All rights reserved.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000455692100010
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166199
Collection中国科学院大连化学物理研究所
Corresponding AuthorShi, Xianzhe
Affiliation1.Southern Univ Sci & Technol, Mat Characterizat & Preparat Ctr, Shenzhen 518055, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Shekou Peoples Hosp, Dept clin lab, Shenzhen 518052, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Hua,Qin, Qian,Shi, Xianzhe,et al. Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1583:80-87.
APA Li, Hua,Qin, Qian,Shi, Xianzhe,He, Jun,&Xu, Guowang.(2019).Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition.JOURNAL OF CHROMATOGRAPHY A,1583,80-87.
MLA Li, Hua,et al."Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition".JOURNAL OF CHROMATOGRAPHY A 1583(2019):80-87.
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