DICP OpenIR
Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants
Du, Nana1,2; Si, Wei1; Liu, Yanfang1; Guo, Zhimou1; Zhou, Weijia1,2; Zhou, Han1; Liang, Xinmiao1; Wang, Chaoran1
Corresponding AuthorLiu, Yanfang(liuyanfang@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
KeywordAlkaloid enrichment Electrostatic repulsion Reversed-phase/strong anion-exchange Scopolia tangutica Mixed-mode stationary phase Class separation
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2019-01-15
ISSN1570-0232
DOI10.1016/j.jchromb.2018.12.024
Volume1105Pages:148-155
Funding ProjectState Key Program of National Natural Science Foundation of China[U1508221] ; National Science Foundation of China[21405155] ; National Science Foundation of China[31670374] ; DICP-CMC innovation institute of medicine
Funding OrganizationState Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLIQUID-CHROMATOGRAPHY ; ACONITUM ALKALOIDS ; ALZHEIMERS-DISEASE ; PHASE ; EXTRACTION ; SEPARATION ; SURFACE ; COLUMN
AbstractSince the content of alkaloids is usually low in plants and they are easily co-eluted with other constituents, enrichment of alkaloids is essential in the discovery of bioactive lead compounds from natural products. In this paper, an easy SPE enrichment method was developed in a buffer-free solvent system based on electrostatic repulsion mechanism. The feasibility of the new method was verified by successful enrichment of alkaloids from Scopolia tangutica (S. tangutica) with an optimized eluting condition. Then this developed method was applied to other representative plants in different families, including Przewalskia tangutica and Peganum harmala L, Lycoris radiata and Menispermum dauricum DC, which enlarged the scope of applicability. Additionally, the new SPE procedure avoided possible structural change destruction caused by pH change. Simple solvent system, including formic acid (FA) and methanol, would benefit subsequent mass analysis, quantity determination and bioactivity screening, and so on.
Language英语
Funding OrganizationState Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine
WOS IDWOS:000456893100017
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166221
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yanfang; Liang, Xinmiao
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Du, Nana,Si, Wei,Liu, Yanfang,et al. Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1105:148-155.
APA Du, Nana.,Si, Wei.,Liu, Yanfang.,Guo, Zhimou.,Zhou, Weijia.,...&Wang, Chaoran.(2019).Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1105,148-155.
MLA Du, Nana,et al."Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1105(2019):148-155.
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