DICP OpenIR
Supramolecular Coordination-Directed Reversible Regulation of Protein Activities at Epigenetic DNA Marks
Wang, Shao-Ru1; Wang, Jia-Qi1; Fu, Bo-Shi2; Chen, Kun1; Xiong, Wei1; Wei, Lai1; Qing, Guangyan3; Tian, Tian1; Zhou, Xiang1
Corresponding AuthorTian, Tian(ttian@whu.edu.cn) ; Zhou, Xiang(xzhou@whu.edu.cn)
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2018-11-21
ISSN0002-7863
DOI10.1021/jacs.8b09113
Volume140Issue:46Pages:15842-15849
Funding ProjectNational Science Foundation of China[21722803] ; National Science Foundation of China[21721005] ; National Science Foundation of China[21572169] ; National Science Foundation of China[21877086] ; National Science Foundation of China[21672165]
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordSTATE KINETIC-ANALYSIS ; THYMINE DNA ; RESTRICTION ENZYME ; STEADY-STATE ; 5-FORMYLCYTOSINE ; POLYMERASE ; BASE ; 5-HYDROXYMETHYLCYTOSINE ; 5-CARBOXYLCYTOSINE ; TRANSCRIPTION
AbstractIn mammals, 5-formylcytosine (5fC) has been identified as an important mark, which plays significant roles in active DNA demethylation and also in epigenetic regulation. It is therefore important to target this epigenetic mark as well as manipulating DNA protein interactions at this site. A unique feature of 5fC is the presence of a formyl group at the C-5 position. In the current study, we introduce supramolecular coordination chemistry for reversible regulation of DNA-protein interactions on this mark. We have designed and synthesized the 2-(aminooxy)-N-(quinolin-8-yl)acetamide (AQA), which functions well in selective labeling of 5fC mark. Using this feature, the association and disassociation of metal ion supplementation allow blocking and deblocking of DNA-protein interactions. In addition, we synthesized a close analogue of AQA by replacing the nitrogen atom in the quinoline ring with a CH group. Importantly, the regulatory effects of those metal ion supplementations were completely erased. On the basis of the combined information, we propose a conformational flexibility in a side arm in response to switched coordination. In the absence of coordinating interaction, the flexible side arm probably takes on an extended conformation and points away from the hydrogen bonding cavity. Importantly, coordinating interaction is effective in imposing a restrained geometry to this side arm, with the quinoline ring being oriented opposite the complementary nucleobase. Moreover, the coordination-induced activity control can be reversed by supplementation with a number of chelating agents. The concept described is unique in installing an auxiliary side arm with bending flexibility to control oligonucleotide functions. Finally, these findings show promising potential of supramolecular coordination chemistry for DNA epigenetics.
Language英语
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China
WOS IDWOS:000451496800042
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166519
Collection中国科学院大连化学物理研究所
Corresponding AuthorTian, Tian; Zhou, Xiang
Affiliation1.Wuhan Univ, Key Lab Biomed Polymers, Minist Educ, Coll Chem & Mol Sci, Wuhan 430072, Hubei, Peoples R China
2.China Med Univ, Dept Pharmacol, Sch Pharm, Shenyang 110122, Liaoning, Peoples R China
3.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shao-Ru,Wang, Jia-Qi,Fu, Bo-Shi,et al. Supramolecular Coordination-Directed Reversible Regulation of Protein Activities at Epigenetic DNA Marks[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2018,140(46):15842-15849.
APA Wang, Shao-Ru.,Wang, Jia-Qi.,Fu, Bo-Shi.,Chen, Kun.,Xiong, Wei.,...&Zhou, Xiang.(2018).Supramolecular Coordination-Directed Reversible Regulation of Protein Activities at Epigenetic DNA Marks.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,140(46),15842-15849.
MLA Wang, Shao-Ru,et al."Supramolecular Coordination-Directed Reversible Regulation of Protein Activities at Epigenetic DNA Marks".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 140.46(2018):15842-15849.
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