DICP OpenIR
Integration of Proteomics and Metabolomics Revealed Metabolite-Protein Networks in ACTH-Secreting Pituitary Adenoma
Feng, Jie1,2,3; Zhang, Qi4; Zhou, Yang5; Yu, Shenyuan1; Hong, Lichuan1; Zhao, Sida1; Yang, Jingjing1; Wan, Hong1; Xu, Guowang5; Zhang, Yazhuo1,2,3; Li, Chuzhong1,2,3
Corresponding AuthorLi, Chuzhong(lichuzhong@163.com)
Keywordmetabolite-protein networks proteomics metabolomics ACTH pituitary adenoma
Source PublicationFRONTIERS IN ENDOCRINOLOGY
2018-11-23
ISSN1664-2392
DOI10.3389/fendo.2018.00678
Volume9Pages:13
Funding ProjectResearch Special Fund for Public Welfare Industry of Health[201402008] ; National High Technology Research and Development Program of China[2014AA020610] ; Beijing Municipal Administration of Hospitals Youth Program[QML20160506] ; National Natural Science Foundation of China[81702455]
Funding OrganizationResearch Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectEndocrinology & Metabolism
WOS Research AreaEndocrinology & Metabolism
WOS KeywordACTIVATION ; PATHWAY ; ROLES ; MYC ; P53
AbstractAn effective treatment for the management of adrenocorticotropic hormone-secreting pituitary adenomas (ACTH-PA) is currently lacking, although surgery is a treatment option. We have integrated information obtained at the metabolomic and proteomic levels to identify critical networks and signaling pathways that may play important roles in the metabolic regulation of ACTH-PA and therefore hopefully represent potential therapeutic targets. Six ACTH-PAs and seven normal pituitary glands were investigated via gas chromatography-mass spectrometry (GC-MS) analysis for metabolomics. Five ACTH-PAs and five normal pituitary glands were subjected to proteomics analysis via nano liquid chromatography tandem-mass spectrometry (nanoLC-MS/MS). The joint pathway analysis and network analysis was performed using MetaboAnalyst 3.0. software. There were significant differences of metabolites and protein expression levels between the ACTH-PAs and normal pituitary glands. A proteomic analysis identified 417 differentially expressed proteins that were significantly enriched in the Myc signaling pathway. The protein-metabolite joint pathway analysis showed that differentially expressed proteins and metabolites were significantly enriched in glycolysis/gluconeogenesis, pyruvate metabolism, citrate cycle (ICA cycle), and the fatty acid metabolism pathway in ACTH-PA. The protein-metabolite molecular interaction network identified from the metabolomics and proteomics investigation resulted in four subnetworks. Ten nodes in subnetwork 1 were the most significantly enriched in cell amino acid metabolism and pyrimidine nucleotide metabolism. Additionally, the metabolite-gene-disease interaction network established nine subnetworks. Ninety-two nodes in subnetwork 1 were the most significantly enriched in carboxylic acid metabolism and organic acid metabolism. The present study clarified the pathway networks that function in ACTH-PA. Our results demonstrated the presence of downregulated glycolysis and fatty acid synthesis in this tumor type. We also revealed that the Myc signaling pathway significantly participated in the metabolic changes and tumorigenesis of ACTH-PA. This data may provide biomarkers for ACTH-PA diagnosis and monitoring, and could also lead to the development of novel strategies for treating pituitary adenomas.
Language英语
Funding OrganizationResearch Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Research Special Fund for Public Welfare Industry of Health ; Research Special Fund for Public Welfare Industry of Health ; National High Technology Research and Development Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Administration of Hospitals Youth Program ; Beijing Municipal Administration of Hospitals Youth Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000451089100001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166541
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Chuzhong
Affiliation1.Capital Med Univ, Beijing Neurosurg Inst, Beijing Tiantan Hosp, Beijing, Peoples R China
2.Capital Med Univ, Beijing Inst Brain Disorders, Brain Tumor Ctr, Beijing, Peoples R China
3.Capital Med Univ, Beijing Tiantan Hosp, China Natl Clin Res Ctr Neurol Dis, Beijing, Peoples R China
4.Zhejiang Univ, Affiliated Hosp 2, Dept Hepatobiliary & Pancreat Surg, Sch Med, Hangzhou, Zhejiang, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Feng, Jie,Zhang, Qi,Zhou, Yang,et al. Integration of Proteomics and Metabolomics Revealed Metabolite-Protein Networks in ACTH-Secreting Pituitary Adenoma[J]. FRONTIERS IN ENDOCRINOLOGY,2018,9:13.
APA Feng, Jie.,Zhang, Qi.,Zhou, Yang.,Yu, Shenyuan.,Hong, Lichuan.,...&Li, Chuzhong.(2018).Integration of Proteomics and Metabolomics Revealed Metabolite-Protein Networks in ACTH-Secreting Pituitary Adenoma.FRONTIERS IN ENDOCRINOLOGY,9,13.
MLA Feng, Jie,et al."Integration of Proteomics and Metabolomics Revealed Metabolite-Protein Networks in ACTH-Secreting Pituitary Adenoma".FRONTIERS IN ENDOCRINOLOGY 9(2018):13.
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