DICP OpenIR
Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay
Wu, Lehao1; Zhang, Weiyue1; Qiu, Xin1; Wang, Chaoran2; Liu, Yanfang2; Wang, Zhiwei3; Yu, Yang1; Ye, Richard D.1,4; Zhang, Yan1
Corresponding AuthorZhang, Yan(zhangyan_sjtu@sjtu.edu.cn)
KeywordCorydalis yanhusuoW. T. Wang luciferase reporter gene assay D-1 receptor FLIPR
Source PublicationMOLECULES
2018-10-01
ISSN1420-3049
DOI10.3390/molecules23102585
Volume23Issue:10Pages:10
Funding ProjectNational Science Foundation of China[81402806] ; National Science Foundation of China[31470865] ; Science and Technology Development Fund of Macau[FDCT 072/2015/A2] ; University of Macau[CPG2015-00018-ICMS]
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordQUATERNARY ALKALOIDS ; DEHYDROCORYDALINE ; ANTINOCICEPTION ; PURIFICATION ; ACTIVATION
AbstractCorydalis yanhusuo W. T. Wang (C. yanhusuo) has been traditionally used for drug addiction and pain relief in China. In our previous study, we showed that the extract of C. yanhusuo blocks dopamine receptors, demonstrating that its pharmacological activities are mostly due to the antagonistic effects of some of its components at dopamine receptors. As part of our ongoing project on C. yanhusuo, the aim of the present study is to establish a high-throughput and low-cost screening assay system and test the abilities of the isolated alkaloids from C. yanhusuo to inhibit dopamine-induced dopamine D-1 receptor activity. By using our established cyclic adenosine monophosphate (cAMP)-response element (CRE)-luciferase reporter gene assay system, we identified eight alkaloids from C. yanhusuo with D-1 receptor antagonistic activities. We next validated the activities of these compounds using fluorometric imaging plate reader (FLIPR) assay by measuring the intracellular Ca2+ change. Six out of eight compounds, including tetrahydropalmatine, corydaline, 13-methyldehydrocorydalmine, dehydrocorybubine, dehydrocorydaline, and columbamine, can be confirmed for their inhibitory activities. The dopamine-receptor-antagonistic effects of four compounds, including 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and corydaline, are reported for the first time. The present study provides an important pharmacological basis to support the traditional use of C. yanhusuo in China.
Language英语
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau
WOS IDWOS:000451201400181
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166563
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Yan
Affiliation1.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.DICP CMC Innovat Inst Med, Taizhou 225300, Peoples R China
4.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China
Recommended Citation
GB/T 7714
Wu, Lehao,Zhang, Weiyue,Qiu, Xin,et al. Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay[J]. MOLECULES,2018,23(10):10.
APA Wu, Lehao.,Zhang, Weiyue.,Qiu, Xin.,Wang, Chaoran.,Liu, Yanfang.,...&Zhang, Yan.(2018).Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay.MOLECULES,23(10),10.
MLA Wu, Lehao,et al."Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay".MOLECULES 23.10(2018):10.
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