DICP OpenIR
Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts
Badiali, Camilla1; De Angelis, Giulia1; Simonetti, Giovanna2; Brasili, Elisa1,3; Tobaruela, Eric de Castro3; Purgatto, Eduardo3; Yin, Heng4; Valletta, Alessio1; Pasqua, Gabriella1
Corresponding AuthorValletta, Alessio(alessio.valletta@uniroma1.it)
KeywordHypericum perforatum Root cultures Chitooligosaccharides Xanthones Volatile organic compounds
Source PublicationPLANT CELL REPORTS
2018-11-01
ISSN0721-7714
DOI10.1007/s00299-018-2317-2
Volume37Issue:11Pages:1471-1484
Funding ProjectSapienza Universita di Roma[C26A15MLP9] ; Sapienza Universita di Roma[AR11715C81A4ABDF]
Funding OrganizationSapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma
WOS SubjectPlant Sciences
WOS Research AreaPlant Sciences
WOS KeywordESSENTIAL OIL COMPOSITION ; CELL-SUSPENSION CULTURES ; HAIRY ROOTS ; L. ; DEATH ; ACCUMULATION ; ELICITORS ; BIOMASS ; GROWTH ; OLIGOCHITOSAN
AbstractKey messageWater-soluble chitosan oligosaccharides (COS) affect xanthone and volatile organic compound content, as well as antifungal activity against human pathogenic fungi of extracts obtained from Hypericum perforatum root cultures.AbstractSeveral studies have demonstrated the elicitor power of chitosan on xanthone biosynthesis in root cultures of H. perforatum. One of the major limitations to the use of chitosan, both for basic and applied research, is the need to use acidified water for solubilization. To overcome this problem, the elicitor effect of water-soluble COS on the biosynthesis of both xanthones and volatile organic compounds (VOCs) was evaluated in the present study. The analysis of xanthones and VOCs was performed by HPLC and GC-MS headspace analysis. The obtained results showed that COS are very effective in enhancing xanthone biosynthesis. With 400mgL(-1) COS, a xanthone content of about 30mgg(-1) DW was obtained. The antifungal activity of extracts obtained with 400mgL(-1) COS was the highest, with MIC50 of 32 mu gmL(-1) against Candida albicans and 32-64 mu g mL(-1) against dermatophytes, depending on the microorganism. Histochemical investigations suggested the accumulation of isoprenoids in the secretory ducts of H. perforatum roots. The presence of monoterpenes and sesquiterpenes was confirmed by the headspace analysis. Other volatile hydrocarbons have been identified. The biosynthesis of most VOCs showed significant changes in response to COS, suggesting their involvement in plant-fungus interactions.
Language英语
Funding OrganizationSapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma
WOS IDWOS:000451470600002
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166599
Collection中国科学院大连化学物理研究所
Corresponding AuthorValletta, Alessio
Affiliation1.Sapienza Univ Rome, Dept Environm Biol, Piazzale Aldo Moro 5, I-00185 Rome, Italy
2.Sapienza Univ Rome, Dept Publ Hlth & Infect Dis, Piazzale Aldo Moro 5, I-00185 Rome, Italy
3.Univ Sao Paulo, Dept Food Sci & Expt Nutr, FORC Food Res Ctr, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo, Brazil
4.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Badiali, Camilla,De Angelis, Giulia,Simonetti, Giovanna,et al. Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts[J]. PLANT CELL REPORTS,2018,37(11):1471-1484.
APA Badiali, Camilla.,De Angelis, Giulia.,Simonetti, Giovanna.,Brasili, Elisa.,Tobaruela, Eric de Castro.,...&Pasqua, Gabriella.(2018).Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts.PLANT CELL REPORTS,37(11),1471-1484.
MLA Badiali, Camilla,et al."Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts".PLANT CELL REPORTS 37.11(2018):1471-1484.
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