DICP OpenIR
Structural insight into precursor tRNA processing by yeast ribonuclease P
Lan, Pengfei1; Tan, Ming2,3; Zhang, Yuebin4; Niu, Shuangshuang2,3,5; Chen, Juan1; Shi, Shaohua1; Qiu, Shuwan6,7; Wang, Xuejuan6,7; Peng, Xiangda4; Cai, Gang6,7; Cheng, Hong2; Wu, Jian1; Li, Guohui4; Lei, Ming1,8,9,10
Corresponding AuthorWu, Jian(wujian@shsmu.edu.cn) ; Li, Guohui(ghli@dicp.ac.cn) ; Lei, Ming(leim@shsmu.edu.cn)
Source PublicationSCIENCE
2018-11-09
ISSN0036-8075
DOI10.1126/science.aat6678
Volume362Issue:6415Pages:657-+
Funding ProjectNational Natural Science Foundation of China[31525007] ; National Natural Science Foundation of China[21625302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010201]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordPRE-TRANSFER-RNA ; DENSITY-FUNCTIONAL THERMOCHEMISTRY ; PARTICLE ELECTRON CRYOMICROSCOPY ; FREE-ENERGY CALCULATIONS ; METAL-BINDING SITE ; MOLECULAR-DYNAMICS ; CRYSTAL-STRUCTURE ; ACTIVE-SITE ; BIOLOGICAL APPLICATIONS ; 2-METAL-ION MECHANISM
AbstractRibonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of Saccharomyces cerevisiae RNase P alone and in complex with pre-tRNA(Phe). The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion S(N)2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences
WOS IDWOS:000450474500033
PublisherAMER ASSOC ADVANCEMENT SCIENCE
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166655
Collection中国科学院大连化学物理研究所
Corresponding AuthorWu, Jian; Li, Guohui; Lei, Ming
Affiliation1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China
2.Chinese Acad Sci, State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai 200031, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
6.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
7.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
8.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China
9.Natl Facil Prot Sci Shanghai, Zhangjiang Lab, Shanghai 201210, Peoples R China
10.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China
Recommended Citation
GB/T 7714
Lan, Pengfei,Tan, Ming,Zhang, Yuebin,et al. Structural insight into precursor tRNA processing by yeast ribonuclease P[J]. SCIENCE,2018,362(6415):657-+.
APA Lan, Pengfei.,Tan, Ming.,Zhang, Yuebin.,Niu, Shuangshuang.,Chen, Juan.,...&Lei, Ming.(2018).Structural insight into precursor tRNA processing by yeast ribonuclease P.SCIENCE,362(6415),657-+.
MLA Lan, Pengfei,et al."Structural insight into precursor tRNA processing by yeast ribonuclease P".SCIENCE 362.6415(2018):657-+.
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