DICP OpenIR
Exchanging the order of carotenogenic genes linked by porcine teschovirus-1 2A peptide enable to optimize carotenoid metabolic pathway in Saccharomyces cerevisiae
Jiao, Xiang1,2; Sun, Wenyi1; Zhang, Yue1; Liu, Xiangjian1; Zhang, Qi1; Wang, Qian1; Zhang, Sufang1,3; Zhao, Zongbao Kent1,3
Corresponding AuthorZhang, Sufang(zsfjxy@dicp.ac.cn)
Source PublicationRSC ADVANCES
2018
ISSN2046-2069
DOI10.1039/c8ra06510a
Volume8Issue:61Pages:34967-34972
Funding ProjectNational Science Foundation of China[31370128] ; National Science Foundation of China[21325627]
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordBETA-CAROTENE ; ESCHERICHIA-COLI ; MULTIPLE PROTEINS ; RHODOSPORIDIUM-TORULOIDES ; YARROWIA-LIPOLYTICA ; CLEAVAGE ACTIVITIES ; EXPRESSION ; ACCUMULATION ; POLYPROTEIN ; APHTHOVIRUS
AbstractThe yeast Saccharomyces cerevisiae serves as a promising host for the production of a wide range of chemical compounds and fuels. Currently, simultaneous expression of several genes could be achieved via the use of 2A viral peptides, yet detailed characterizations to assess the discrepancy of different orders of genes linked by 2A peptides are rarely sufficient. In this study, we investigated the effects of the order of genes linked by porcine teschovirus-1 2A (P2A) peptide on the metabolic pathway in S. cerevisiae. A heterologous carotenoid biosynthetic system involving nine kinds of polycistronic expression of codon-optimized carotenogenic genes GGPPS, CARB and CARRP from Blakeslea trispora was introduced into S. cerevisiae. The order of genes in the polycistronic segment was exchanged; -carotene production by engineered yeasts was significantly different. The highest -carotene yield was achieved in transformants carrying the plasmid, with CARB as the first gene in the polycistronic construct regardless of the location of GGPPS, CARRP. In addition, we found that -carotene production was coupled with the growth in engineered strain with the highest -carotene content during the shake flask fermentation and fed-batch fermentation. A novel microbial heterologous carotenoid production system was established by optimizing the order of genes linked by P2A peptide sequences in a polycistronic expression construct. The observation of the importance of the order in a polycistronic construct may be used to increase yields in other P2A peptide-containing expression systems.
Language英语
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China
WOS IDWOS:000448348600025
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166739
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Sufang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Jiao, Xiang,Sun, Wenyi,Zhang, Yue,et al. Exchanging the order of carotenogenic genes linked by porcine teschovirus-1 2A peptide enable to optimize carotenoid metabolic pathway in Saccharomyces cerevisiae[J]. RSC ADVANCES,2018,8(61):34967-34972.
APA Jiao, Xiang.,Sun, Wenyi.,Zhang, Yue.,Liu, Xiangjian.,Zhang, Qi.,...&Zhao, Zongbao Kent.(2018).Exchanging the order of carotenogenic genes linked by porcine teschovirus-1 2A peptide enable to optimize carotenoid metabolic pathway in Saccharomyces cerevisiae.RSC ADVANCES,8(61),34967-34972.
MLA Jiao, Xiang,et al."Exchanging the order of carotenogenic genes linked by porcine teschovirus-1 2A peptide enable to optimize carotenoid metabolic pathway in Saccharomyces cerevisiae".RSC ADVANCES 8.61(2018):34967-34972.
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