DICP OpenIR
A 3D human lung-on-a-chip model for nanotoxicity testing
Zhang, Min1; Xu, Cong1,2; Jiang, Lei1; Qin, Jianhua1,2,3,4
Corresponding AuthorQin, Jianhua(jhqin@dicp.ac.cn)
Source PublicationTOXICOLOGY RESEARCH
2018-11-01
ISSN2045-452X
DOI10.1039/c8tx00156a
Volume7Issue:6Pages:13
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDPB0305] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020900] ; Key Program of the Chinese Academy of Sciences[KFZD-SW-213] ; National Key R&D Program of China[2017YFB0405400] ; International Science & Technology Cooperation Program of China[2015DFA00740] ; National Nature Science Foundation of China[91543121] ; National Nature Science Foundation of China[81573394] ; National Nature Science Foundation of China[31671038] ; Innovation Program of Science and Research from the DICP, CAS[DICP TMSR201601]
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
WOS SubjectToxicology
WOS Research AreaToxicology
WOS KeywordMETAL-OXIDE NANOPARTICLES ; IN-VITRO ; ENGINEERED NANOPARTICLES ; TOXICITY ASSESSMENT ; PULMONARY TOXICITY ; EPITHELIAL-CELLS ; CELLULAR TOXICITY ; OXIDATIVE STRESS ; BARRIER FUNCTION ; SHEAR-STRESS
AbstractThe prevalent application of nanoparticles (NPs) has drawn intense concerns about their impact on the environment and human health. Inhalation of NPs is the major route of NP exposure and has led to adverse effects on the lung. It is of great concern to evaluate the potential hazards of nanoparticles for human health during pulmonary exposure. Here, we proposed a novel 3D human lung-on-a-chip model to recreate the organ-level structure and functions of the human lung that allow to us evaluate the pulmonary toxicity of nanoparticles. The lung-on-a-chip consists of three parallel channels for the co-culture of human vascular endothelial cells and human alveolar epithelial cells sandwiching a layer of Matrigel membrane, which recapitulate the key features of the alveolar capillary barrier in the human lung. Cell-cell interaction, cell-matrix interaction and vascular mechanical cues work synergistically to promote the barrier function of the lung-on-a-chip model. TiO2 nanoparticles and ZnO nanoparticles were applied on the lung-on-a-chip to assay their nanotoxicity on both epithelial cells and endothelial cells. Junction protein expression, increased permeability to macromolecules, dose dependent cytotoxicity, ROS production and apoptosis were assayed and compared on the chip. This lung-on-a-chip model indicated its versatile application in human pulmonary health and safety assessment for nanoparticles, environment, food and drugs.
Language英语
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
WOS IDWOS:000448823300006
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166783
Collection中国科学院大连化学物理研究所
Corresponding AuthorQin, Jianhua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Div Biotechnol, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China
4.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Min,Xu, Cong,Jiang, Lei,et al. A 3D human lung-on-a-chip model for nanotoxicity testing[J]. TOXICOLOGY RESEARCH,2018,7(6):13.
APA Zhang, Min,Xu, Cong,Jiang, Lei,&Qin, Jianhua.(2018).A 3D human lung-on-a-chip model for nanotoxicity testing.TOXICOLOGY RESEARCH,7(6),13.
MLA Zhang, Min,et al."A 3D human lung-on-a-chip model for nanotoxicity testing".TOXICOLOGY RESEARCH 7.6(2018):13.
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