DICP OpenIR
Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment
Wang, Shuyan1,2; Meng, Yingying2,3; Liu, Jiao2,4; Cao, Xupeng2; Xue, Song2
Corresponding AuthorXue, Song(xuesong@dicp.ac.cn)
KeywordQuantification of astaxanthin Haematococcus pluvialis Dimethyl sulfoxide extraction Cholesterol esterase hydrolysis
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
2018-11-01
ISSN2211-9264
DOI10.1016/j.algal.2018.08.029
Volume35Pages:427-431
Funding ProjectEducational Department of Inner Mongolia[NJZY168]
Funding OrganizationEducational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordMICROALGAE ; ESTERS ; ACCUMULATION
AbstractHaematococcus pluvialis accumulates high contents of astaxanthin and is considered as an important source of natural astaxanthin production. Fast and accurate quantification of astaxanthin is needed to evaluate the quality of microalgal sources for astaxanthin extraction and to monitor the H. pluvialis cultivation process. In the present study, a less-invasive method for astaxanthin accurate quantification was developed. Dimethyl sulfoxide (DMSO) was used as an extraction solvent to improve the membrane permeability of H. pluvialis. Cholesterol esterase was used to hydrolyze astaxanthin esters via incubation at 36 degrees C for 30 min. The detection of astaxanthin after hydrolysis was achieved by high performance liquid chromatography (HPLC) within 10 min using a C18 column. The astaxanthin content measurements after enzymatic pretreatment were 1.5-1.7 times higher than that measured after saponified pretreatment. DMSO solvent extraction combined with lipase-catalyzed hydrolysis followed by HPLC detection is proposed for the accurate quantification of astaxanthin in H. pluvialis.
Language英语
Funding OrganizationEducational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia
WOS IDWOS:000447187700046
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166891
Collection中国科学院大连化学物理研究所
Corresponding AuthorXue, Song
Affiliation1.Inner Mongolia Univ Nationlities, Chem & Chem Engn Coll, Tongliao 028000, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
3.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China
4.Qilu Univ Technol, Dept Bioengn, Jinan 250353, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shuyan,Meng, Yingying,Liu, Jiao,et al. Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,35:427-431.
APA Wang, Shuyan,Meng, Yingying,Liu, Jiao,Cao, Xupeng,&Xue, Song.(2018).Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,35,427-431.
MLA Wang, Shuyan,et al."Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 35(2018):427-431.
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