DICP OpenIR
Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish
Zhao, Fa1; Liu, Yanming1; Zhang, Xiqi1; Dong, Rui1; Yu, Wenjiang1; Liu, Yanfang2; Guo, Zhimou2; Liang, Xinmiao2; Zhu, Jianhua1
Corresponding AuthorLiu, Yanming(msymliu@163.com)
KeywordFish Arsenic species Protease Ammonium carbonate Kinetic energy discrimination HPLC-ICP-MS
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2018-10-26
ISSN0021-9673
DOI10.1016/j.chroma.2018.08.068
Volume1573Pages:48-58
Funding ProjectNational Key Research and Development Program of China[2017YFC1601600]
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordATOMIC-ABSORPTION-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; HPLC-ICP-MS ; MULTIELEMENTAL SPECIATION ANALYSIS ; WATER SAMPLES ; FLUORESCENCE SPECTROMETRY ; DRINKING-WATER ; SEPARATION ; SEAFOOD ; SELENIUM
AbstractA sensitive, simple and rapid method for the simultaneous determination of eleven arsenic species has been developed. A high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) technique was used for the analysis of eleven arsenic species in less than 17 min. Different extraction solutions were explored and the recovery results using water and aqueous acidic solvents, aqueous organic solvents and enzymes showed that 40 mg protease with 0.75 mL 0.5% hydrochloric acid (v/v) as the extraction agent gave the best experimental results. Species separation with ammonium carbonate solution as the mobile phase was conducted on an anion-exchange chromatographic column using gradient elution. The column temperature was 20 degrees C and kinetic energy discrimination (KED) was employed to eliminate spectral interference. The use of KED mode effectively removed interference from (ArCl)-Ar-75. The detection limit (L-D) of the method was in the range of 0.11-0.59 mu g kg(-1). Repeatability values obtained for spiked real fish samples were in the range of 1.1%-7.6%. Accuracy was calculated based on the analysis of spiked real fish samples at five concentration levels. Obtained recoveries were 91%-106%. The validated method was used in a pilot study to analyze real samples of fish, the organic arsenic especially AsB was the major arsenic specie present in the analyzed samples, only trace amount of inorganic arsenic were detected. The analytical method should improve the assessment of human exposure associated with arsenic intake from fish. (C) 2018 Elsevier B.V. All rights reserved.
Language英语
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China
WOS IDWOS:000447572400005
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166941
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yanming
Affiliation1.Shandong Inst Food & Drug Control, Jinan 250100, Shandong, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Fa,Liu, Yanming,Zhang, Xiqi,et al. Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1573:48-58.
APA Zhao, Fa.,Liu, Yanming.,Zhang, Xiqi.,Dong, Rui.,Yu, Wenjiang.,...&Zhu, Jianhua.(2018).Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish.JOURNAL OF CHROMATOGRAPHY A,1573,48-58.
MLA Zhao, Fa,et al."Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish".JOURNAL OF CHROMATOGRAPHY A 1573(2018):48-58.
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