DICP OpenIR
Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis
Han, Shuai1; Yang, Kaiguang2; Zhu, Hong1; Liu, Jianhui2; Zhang, Lihua2; Zhao, Jiuyang3
Corresponding AuthorZhang, Lihua(lihuazhang@dicp.ac.cn) ; Zhao, Jiuyang(zhaojiuy9@163.com)
KeywordProteomics serum proteins hemodialysis dialyzer
Source PublicationRENAL FAILURE
2018-10-03
ISSN0886-022X
DOI10.1080/0886022X.2018.1491406
Volume40Issue:1Pages:506-513
WOS SubjectUrology & Nephrology
WOS Research AreaUrology & Nephrology
WOS KeywordCHRONIC KIDNEY-DISEASE ; TANDEM MASS-SPECTROMETRY ; D-BINDING PROTEIN ; ONLINE HEMODIAFILTRATION ; DIALYSIS MEMBRANES ; NEPHROTIC SYNDROME ; OXIDATIVE STRESS ; STABLE-ISOTOPE ; ADSORPTION ; HDL
AbstractPurpose: This study aimed to use proteomics methods to investigate the changes in serum protein levels after high- and low-flux hemodialysis (HD). Methods: Before and after HD, serum samples were obtained from two selected patients who were treated with a Polyflux 140H high-flux dialyzer and a Polyflux 14L low-flux dialyzer during two continuous therapy sessions. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed to identify the proteins. Results: A total of 212 and 203 serum proteins were identified after high-flux and low-flux HD, respectively. After high-flux HD, 21 proteins increased, and 132 proteins decreased. After low-flux HD, 87 proteins increased, and 45 proteins decreased. High-flux HD led to a significantly greater reduction in protein levels than low-flux HD (0.73 +/- 0.13 vs. 0.84 +/- 0.18, p = .00). Among the increased and decreased proteins, the isoelectric point (pI) values mainly ranged from 5 to 7, and the molecular weights (Mws) were mostly smaller than 30 kDa. The serum proteins showed no difference in pI or Mw for high- and low-flux HD. Gene ontology (GO) analysis showed that the detected proteins were related to immune system processes and complement activation. Conclusions: Serum protein levels differentially changed after high- and low-flux HD. Long-term effects should be observed in future studies.
Language英语
WOS IDWOS:000446358100001
PublisherTAYLOR & FRANCIS LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/166967
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua; Zhao, Jiuyang
Affiliation1.Dalian Univ, Affiliated Zhongshan Hosp, Dalian, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Nephrit Med, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Han, Shuai,Yang, Kaiguang,Zhu, Hong,et al. Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis[J]. RENAL FAILURE,2018,40(1):506-513.
APA Han, Shuai,Yang, Kaiguang,Zhu, Hong,Liu, Jianhui,Zhang, Lihua,&Zhao, Jiuyang.(2018).Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis.RENAL FAILURE,40(1),506-513.
MLA Han, Shuai,et al."Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis".RENAL FAILURE 40.1(2018):506-513.
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