DICP OpenIR
Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm
Wei, Jiangbo1,2,3; Liu, Fange1,2,3; Lu, Zhike1,2,3,4; Fei, Qili1,2,3; Ai, Yuxi1,2,3; He, P. Cody1,2,3; Shi, Hailing1,2,3; Cui, Xiaolong1,2,3; Su, Rui5; Klungland, Arne6; Jia, Guifang7; Chen, Jianjun5; He, Chuan1,2,3
Corresponding AuthorHe, Chuan(chuanhe@uchicago.edu)
Source PublicationMOLECULAR CELL
2018-09-20
ISSN1097-2765
DOI10.1016/j.molcel.2018.08.011
Volume71Issue:6Pages:973-+
Funding ProjectU.S. National Insititutes of Health[GM071440] ; U.S. National Insititutes of Health[HG008935] ; U.S. National Insititutes of Health[CA214965] ; National Basic Research Program of China[2014CB964900] ; U.S. National Science Foundation[CHE-1048528] ; U.S. National Institutes of Health[CA014599]
Funding OrganizationU.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS KeywordOBESITY-ASSOCIATED FTO ; PRE-MESSENGER-RNA ; SUBSTRATE-SPECIFICITY ; CRYSTAL-STRUCTURES ; METHYLATION ; GENE ; N6-METHYLADENOSINE ; TRANSLATION ; REVEALS ; ALKB
AbstractFTO, the first RNA demethylase discovered, mediates the demethylation of internal N-6-methyladenosine (m(6)A) and N-6, 2-O-dimethyladenosine (m(6)A(m)) at the +1 position from the 5' cap in mRNA. Here we demonstrate that the cellular distribution of FTO is distinct among different cell lines, affecting the access of FTO to different RNA substrates. We find that FTO binds multiple RNA species, including mRNA, snRNA, and tRNA, and can demethylate internal m(6)A and cap m(6)A(m) in mRNA, internal m(6)A in U6 RNA, internal and cap m(6)A(m) in snRNAs, and N-1-methyladenosine (m(1)A) in tRNA. FTO-mediated demethylation has a greater effect on the transcript levels of mRNAs possessing internal m(6)A than the ones with cap m(6)A(m) in the tested cells. We also show that FTO can directly repress translation by catalyzing m(1)A tRNA demethylation. Collectively, FTO-mediated RNA demethylation occurs to m(6)A and m(6)A(m) in mRNA and snRNA as well as m(1)A in tRNA.
Language英语
Funding OrganizationU.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health
WOS IDWOS:000445103900010
PublisherCELL PRESS
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167041
Collection中国科学院大连化学物理研究所
Corresponding AuthorHe, Chuan
Affiliation1.Univ Chicago, Dept Biochem & Mol Biol, Dept Chem, 929 East 57 St, Chicago, IL 60637 USA
2.Univ Chicago, Inst Biophys Dynam, 929 East 57 St, Chicago, IL 60637 USA
3.Univ Chicago, Howard Hughes Med Inst, 929 East 57 St, Chicago, IL 60637 USA
4.Westlake Univ, Westlake Inst Adv Study, Inst Nat Sci, 18 Shilongshan Rd, Hangzhou 310064, Zhejiang, Peoples R China
5.Beckman Res Inst City Hope, Dept Syst Biol, Monrovia, CA 91016 USA
6.Univ Oslo, Norway Inst Basic Med Sci, Inst Med Microbiol, Oslo Univ Hosp,Rikshosp, POB 1018 Blindern, N-0315 Oslo, Norway
7.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci,Minist Educ,Coll Chem &, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wei, Jiangbo,Liu, Fange,Lu, Zhike,et al. Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm[J]. MOLECULAR CELL,2018,71(6):973-+.
APA Wei, Jiangbo.,Liu, Fange.,Lu, Zhike.,Fei, Qili.,Ai, Yuxi.,...&He, Chuan.(2018).Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm.MOLECULAR CELL,71(6),973-+.
MLA Wei, Jiangbo,et al."Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm".MOLECULAR CELL 71.6(2018):973-+.
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