DICP OpenIR
Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase
Li, Kuikui; Chen, Wei; Wang, Wenxia; Tan, Haidong; Li, Shuguang; Yin, Heng
Corresponding AuthorYin, Heng(yinheng@dicp.ac.cn)
Keywordbeta-1 -> Curdlan powder Pre-treatment Glucan oligosaccharide Degradation mode 3-Glucanase
Source PublicationCARBOHYDRATE POLYMERS
2018-12-01
ISSN0144-8617
DOI10.1016/j.carbpol.2018.08.048
Volume201Pages:122-130
Funding ProjectNational Key R&D Programme of China[2017YFD0200900] ; Shaanxi Provincial Key RD Programme[2017TSCXL-NY-02-05] ; National Natural Science Foundation of China[31670803] ; Dalian City Innovative Support Programme for High-Level Talents[2015R010] ; CAS Youth Innovation Promotion Association[2015144]
Funding OrganizationNational Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS Research AreaChemistry ; Polymer Science
WOS KeywordEXTRACELLULAR POLYSACCHARIDES ; ESSENTIAL-RESIDUES ; ATCC 31749 ; OLIGOSACCHARIDES ; BETA-1,3-GLUCAN ; ENDO-BETA-1,3-GLUCANASE ; PURIFICATION ; SPECIFICITY ; HYDROLYSIS ; RHIZOBIUM
AbstractCurdlan is a water-insoluble microbial exo-polysaccharide that is hardly degraded. The gene CcGluE encoding an endo-beta-1 -> 3-glucanase consisting of 412 amino acids (44 kDa) from Cellulosimicrobium cellulans E4-5 was cloned and expressed in Escherichia coll. The recombinant CcGluE hydrolysed curdlan powder effectively. CcGluE shows high endo-beta-1 -> 3 glucanase activity and low beta-1,4 and beta-1,6 glucanase activities with broad substrate specificity for glucan, including curdlan, laminarin and beta-1 -> 3/1 -> 6-glucan, and the highest catalytic activity for curdlan. Moreover, the hydrolytic products of curdlan were glucan oligosaccharides with degrees of polymerisation of 2-13, and the main products were glucobiose and glucotriose. Degradation mode analysis indicated that CcGluE is more likely to hydrolyse glucopentaose and revealed that CcGluE was an endo-glucanase. Furthermore, upon combination with a homogenising pre-treatment method with curdlan, the degradation efficiency of CcGluE for curdlan powder was greatly improved 7.1-fold, which laid a good foundation for the utilisation of curdlan.
Language英语
Funding OrganizationNational Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association
WOS IDWOS:000445030100014
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167053
Collection中国科学院大连化学物理研究所
Corresponding AuthorYin, Heng
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Kuikui,Chen, Wei,Wang, Wenxia,et al. Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase[J]. CARBOHYDRATE POLYMERS,2018,201:122-130.
APA Li, Kuikui,Chen, Wei,Wang, Wenxia,Tan, Haidong,Li, Shuguang,&Yin, Heng.(2018).Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase.CARBOHYDRATE POLYMERS,201,122-130.
MLA Li, Kuikui,et al."Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase".CARBOHYDRATE POLYMERS 201(2018):122-130.
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