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The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii
Zhu,Zhen1; Yuan,Guangze1; Fan,Xuran2; Fan,Yan2,3; Yang,Miao2,3; Yin,Yalei4; Liu,Jiao2; Liu,Yang4; Cao,Xupeng2; Tian,Jing1; Xue,Song2
Corresponding AuthorCao,Xupeng(c_x_p@dicp.ac.cn) ; Tian,Jing(tianjing@dlpu.edu.cn) ; Xue,Song(xuesong@dicp.ac.cn)
KeywordPhospholipid: diacylglycerol acyltransferase PDAT Synchronous TAG production Chlamydomonas reinhardtii Lipid
Source PublicationBiotechnology for Biofuels
2018-06-06
ISSN1754-6834
DOI10.1186/s13068-018-1160-6
Volume11Issue:1
AbstractAbstractBackgroundThe synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in Chlamydomonas reinhardtii.ResultsUsing a chimeric Hsp70A–RbcS2 promoter, exogenous PDAT form Saccharomyces cerevisiae fused with a chloroplast transit peptide was expressed in C. reinhardtii CC-137. Proved by western blot, the expression of ScPDAT showed a synchronous trend to the growth in the exponential phase. Compared to the wild type, the strain of Scpdat achieved 22% increase in the content of total fatty acids and 32% increase in TAG content. In addition, the fluctuation of C16 series fatty acid in monogalactosyldiacylglycerol, diacylglyceryltrimethylhomoserine and TAG indicated an enhancement in the TAG accumulation pathway.ConclusionThe TAG production was enhanced in the regular cultivation without the nutrient stress by strengthening the conversion of polar lipid to TAG in C. reinhardtii and the findings provide a candidate strategy for rational engineered strain to overcome the decline in the growth during the TAG accumulation triggered by nitrogen starvation.
Language英语
WOS IDBMC:10.1186/s13068-018-1160-6
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167089
Collection中国科学院大连化学物理研究所
Corresponding AuthorCao,Xupeng; Tian,Jing; Xue,Song
Affiliation1.
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Recommended Citation
GB/T 7714
Zhu,Zhen,Yuan,Guangze,Fan,Xuran,et al. The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii[J]. Biotechnology for Biofuels,2018,11(1).
APA Zhu,Zhen.,Yuan,Guangze.,Fan,Xuran.,Fan,Yan.,Yang,Miao.,...&Xue,Song.(2018).The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii.Biotechnology for Biofuels,11(1).
MLA Zhu,Zhen,et al."The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii".Biotechnology for Biofuels 11.1(2018).
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