Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells
Liu,Chang1,2; Liu,Yang1; Xu,Xiao-xi1,2; Guo,Xin1; Sun,Guang-wei1; Ma,Xiao-jun1
Corresponding AuthorSun,Guang-wei(sungw@dicp.ac.cn)
KeywordThree-dimensional cell culture Umbilical cord mensenchymal stem cells Hepatocellular carcinoma Metastasis TGF-β
Source PublicationBMC Cancer
AbstractAbstractBackgroundAccumulating evidences have demonstrated that mesenchymal stem cells (MSC) could be recruited to the tumor microenvironment. Umbilical cord mesenchymal stem cells (UCMSC) were attractive vehicles for delivering therapeutic agents against cancer. Nevertheless, the safety of UCMSC in the treatment of tumors including hepatocellular carcinoma (HCC) was still undetermined.MethodsIn this study, an in vitro co-culture system was established to evaluate the effect of UCMSC on the cell growth, cancer stem cell (CSC) characteristics, drug resistance, metastasis of 3D-cultured HCC cells, and the underlying mechanism was also investigated.ResultsIt was found that after co-cultured with UCMSC, the metastatic ability of 3D-cultured HCC cells was significantly enhanced as indicated by up-regulation of matrix metalloproteinase (MMP), epithelial-mesenchymal transition (EMT)-related genes, and migration ability. However, cell growth, drug resistance and CSC-related gene expression of HCC cells were not affected by UCMSC. Moreover, EMT was reversed, MMP-2 expression was down-regulated, and migration ability of HCC cell was significantly inhibited when TGF-β receptor inhibitor SB431542 was added into the co-culture system.ConclusionsTherefore, these data indicated that UCMSC could significantly enhance the tumor cell metastasis, which was due to the EMT of HCC cells induced by TGF-β.
WOS IDBMC:10.1186/s12885-016-2595-4
PublisherBioMed Central
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Document Type期刊论文
Corresponding AuthorSun,Guang-wei
Affiliation1.Laboratory of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
2.University of Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu,Chang,Liu,Yang,Xu,Xiao-xi,et al. Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells[J]. BMC Cancer,2016,16(1).
APA Liu,Chang,Liu,Yang,Xu,Xiao-xi,Guo,Xin,Sun,Guang-wei,&Ma,Xiao-jun.(2016).Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells.BMC Cancer,16(1).
MLA Liu,Chang,et al."Mesenchymal stem cells enhance the metastasis of 3D-cultured hepatocellular carcinoma cells".BMC Cancer 16.1(2016).
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