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Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis
Bai, Ruihan1,2; Li, Linmei2; Liu, Meimei2; Yan, Shiqiang2; Miao, Chunyue1,2; Li, Ruijun1; Luo, Yong3,4; Liu, Tingjiao5; Lin, Bingcheng2; Ji, Yibing1; Lu, Yao2
Source PublicationANALYTICAL CHEMISTRY
2018-05-01
ISSN0003-2700
DOI10.1021/acs.analchem.8b00362
Volume90Issue:9Pages:5825-5832
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordDRUG DISCOVERY ; T-CELLS ; CULTURE ; HETEROGENEITY ; CYTOMETRY ; PLATFORM ; MIGRATION ; RESPONSES ; PROTEINS ; MATRICES
AbstractDespite rapid progresses in single-cell analysis technologies, efforts to control the three-dimensional micro-environment for single cell measurements have been lacking. Here, we report a simple method to incorporate three-dimensional scaffolds, including polyvinylidene fluoride (PVDF) membranes and PVDF membrane replicated analog polydimethylsiloxane, into multiplexed single cell secretomic analysis platforms (including a microwell array and a single cell barcode microchip) to mimic the extracellular physical matrix and mechanical support for single cells. Applying this platform to brain tumor cell line U87 to investigate single cell protein secretion behavior on different substrates, we revealed that single cell protein secretions were regulated differently in three-dimensional (3D) microenvironments. This finding was further verified with intracellular cytokine staining, highlighting the significance of 3D single cell biomimetic platform can be easily adaptable to other three-dimensional cell culture scaffolds or other single cell assays and may become a broadly applicable three-dimensional single cell analysis system to study the effect of microenvironment conditions on cellular functional heterogeneity in vitro.
Language英语
WOS IDWOS:000431464400043
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167155
Collection中国科学院大连化学物理研究所
Corresponding AuthorJi, Yibing; Lu, Yao
Affiliation1.China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China
3.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116024, Liaoning, Peoples R China
4.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Liaoning, Peoples R China
5.Dalian Med Univ, Coll Stomatol, Dalian 116044, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Bai, Ruihan,Li, Linmei,Liu, Meimei,et al. Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis[J]. ANALYTICAL CHEMISTRY,2018,90(9):5825-5832.
APA Bai, Ruihan.,Li, Linmei.,Liu, Meimei.,Yan, Shiqiang.,Miao, Chunyue.,...&Lu, Yao.(2018).Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis.ANALYTICAL CHEMISTRY,90(9),5825-5832.
MLA Bai, Ruihan,et al."Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis".ANALYTICAL CHEMISTRY 90.9(2018):5825-5832.
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