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Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp.
Yu, Zuochen1,2; Zhu, Benwei3; Wang, Wenxia1; Tan, Haidong1; Yin, Heng1
KeywordAlginate Monosaccharide Oligoalginate Lyase
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2018-06-01
ISSN0141-8130
DOI10.1016/j.ijbiomac.2018.02.046
Volume112Pages:937-942
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS KeywordALGINATE LYASE ; AGROBACTERIUM-TUMEFACIENS ; MOLECULAR-IDENTIFICATION ; MAJOR SOURCES ; ACID ; DEPOLYMERIZATION ; METABOLISM ; ATU3025
AbstractA new oligoalginate lyase encoding gene, designed oal17A, was cloned from marine bacterium Vibrio sp. W13, and then expressed in Escherichia coli. The recombinant Oal17A was purified by NTA-Ni resin with maximal activity at 30 degrees C and pH 7.0. Oal17A exhibited broad substrate specificity, and preferred to degrade alginate than polyM or polyG into monosaccharide acid. The specific activity of Oal17A toward alginate, polyM and polyG was 21.14 U/mg, 12.31 U/mg and 7.43 U/mg, respectively. With features of high-level expression and broad substrate specificity, Oal17A would be a potential tool for alginate monomer production process of alginate utilizing for biofuels and bioethanol production. (C) 2018 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000430522400110
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167207
Collection中国科学院大连化学物理研究所
Corresponding AuthorYin, Heng
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Yu, Zuochen,Zhu, Benwei,Wang, Wenxia,et al. Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp.[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,112:937-942.
APA Yu, Zuochen,Zhu, Benwei,Wang, Wenxia,Tan, Haidong,&Yin, Heng.(2018).Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp..INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,112,937-942.
MLA Yu, Zuochen,et al."Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp.".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 112(2018):937-942.
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