DICP OpenIR
Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities
Wang, Anqi1,3; Feng, Jiesi2,3; Li, Yulong2,3; Zou, Peng1,3
KeywordBiosensors Protein Engineering Fluorescence Imaging Bioluminescence
Source PublicationACS CHEMICAL NEUROSCIENCE
2018-04-01
ISSN1948-7193
DOI10.1021/acschemneuro.7b00455
Volume9Issue:4Pages:639-650
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Medicinal ; Neurosciences
WOS Research AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Neurosciences & Neurology
WOS KeywordRESONANCE ENERGY-TRANSFER ; SINGLE-CELL LEVEL ; VOLTAGE-SENSOR ; LIVING CELLS ; IN-VIVO ; NITRIC-OXIDE ; SYNAPTIC-TRANSMISSION ; HYDROGEN-PEROXIDE ; SNAP-TAG ; BIOLUMINOGENIC PROBE
AbstractOptical biosensors have been invaluable tools in neuroscience research, as they provide the ability to directly visualize neural activity in real time, with high specificity, and with exceptional spatial and temporal resolution. Notably, a majority of these sensors are based on fluorescent protein scaffolds, which offer the ability to target specific cell types or even subcellular compartments. However, fluorescent proteins are intrinsically bulky tags, often insensitive to the environment, and always require excitation light illumination. To address these limitations, there has been a proliferation of alternative sensor scaffolds developed in recent years, including hybrid sensors that combine the advantages of synthetic fluorophores and genetically encoded protein tags, as well as bioluminescent probes. While still in their early stage of development as compared with fluorescent protein-based sensors, these novel probes have offered complementary solutions to interrogate various aspects of neuronal communication, including transmitter release, changes in membrane potential, and the production of second messengers. In this Review, we discuss these important new developments with a particular focus on design strategies.
Language英语
WOS IDWOS:000430642400005
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167225
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Yulong; Zou, Peng
Affiliation1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Synthet & Funct Biomol Ctr,Minist Educ, Beijing Natl Lab Mol Sci,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100871, Peoples R China
3.Peking Univ, PKU IDG McGovern Inst Brain Res, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wang, Anqi,Feng, Jiesi,Li, Yulong,et al. Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities[J]. ACS CHEMICAL NEUROSCIENCE,2018,9(4):639-650.
APA Wang, Anqi,Feng, Jiesi,Li, Yulong,&Zou, Peng.(2018).Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities.ACS CHEMICAL NEUROSCIENCE,9(4),639-650.
MLA Wang, Anqi,et al."Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities".ACS CHEMICAL NEUROSCIENCE 9.4(2018):639-650.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Anqi]'s Articles
[Feng, Jiesi]'s Articles
[Li, Yulong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Anqi]'s Articles
[Feng, Jiesi]'s Articles
[Li, Yulong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Anqi]'s Articles
[Feng, Jiesi]'s Articles
[Li, Yulong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.