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Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid
Chang, Cuilan; Zhou, Zhigui; Yang, Youyou; Han, Yehua; Bai, Yu; Zhao, Meiping; Liu, Huwei
KeywordChiral Separation Dart-ms Jasmonic Acid 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol Nplc
Source PublicationELECTROPHORESIS
2012-11-01
ISSN0173-0835
DOI10.1002/elps.201200122
Volume33Issue:22Pages:3387-3393
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ATMOSPHERIC-PRESSURE PHOTOIONIZATION ; DART-MS ; ELECTROSPRAY-IONIZATION ; HUMAN PLASMA ; ION-SOURCE ; S-OXIDE ; RESOLUTION ; PHARMACEUTICALS
AbstractNormal phase chiral LC (NPLC) has been proved to be powerful and efficient for chiral separation. However, the combination of NPLC with ESI or atmospheric pressure chemical ionization MS is restricted by the poor ionization efficiency and thermal fragmentations of analytes to some extent. Direct analysis in real time MS (DART-MS) is an ambient ionization technique that shows high ionization efficiency of the analytes in the normal phase mobile phase. In this work, we coupled chiral NPLC to DART-MS for the chiral qualitative and quantitative analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid enantiomers. Satisfactory results for the enantiomers of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol operating in the positive mode were obtained in terms of linearity (2.5250 mu g/mL, R-2, 0.999-1.000) and repeatability (25 mu g/mL, RSDs, 4.7-5.6%). Moreover, chiral NPLC-DART-MS resulted in the simultaneous chiral separation and detection of jasmonic acid enantiomers, which are very difficult to be analyzed by NPLC-ESI-MS and NPLC-APCI-MS. Compared with the coupled techniques of NPLC-ESI-MS and NPLC-APCI-MS, NPLC-DART-MS showed advantages in increasing the ionization efficiency and reducing the in-source thermal fragmentation of analytes.
Language英语
WOS IDWOS:000311303100018
PublisherWILEY-BLACKWELL
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167275
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Huwei
AffiliationPeking Univ, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn,Inst Analyt Chem,Minist Educ, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Chang, Cuilan,Zhou, Zhigui,Yang, Youyou,et al. Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid[J]. ELECTROPHORESIS,2012,33(22):3387-3393.
APA Chang, Cuilan.,Zhou, Zhigui.,Yang, Youyou.,Han, Yehua.,Bai, Yu.,...&Liu, Huwei.(2012).Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid.ELECTROPHORESIS,33(22),3387-3393.
MLA Chang, Cuilan,et al."Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid".ELECTROPHORESIS 33.22(2012):3387-3393.
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