DICP OpenIR
Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling
Zheng, Siqi1; Zhang, Gong2; Li, Jie1; Chen, Peng R.1,2
KeywordAnthrax Lethal Factor Bioorthogonal Reactions Genetic Code Expansion Membrane Translocation Protein Labeling
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
2014-06-01
ISSN1433-7851
DOI10.1002/anie.201403945
Volume53Issue:25Pages:6449-6453
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordAZIDE-ALKYNE CYCLOADDITION ; TOXIN TRANSLOCATION ; FLUORESCENT PROTEIN ; CRYSTAL-STRUCTURE ; PORE ; RECEPTOR ; LONG
AbstractCoupling the genetic code expansion technique with bioorthogonal reactions enables precise control over the conjugation site as well as the choice of fluorescent probes during protein labeling. However, the advantages of this strategy over bulky and rigid fluorescent proteins (FPs) remain to be fully explored. Here we applied site-specific bioorthogonal labeling on anthrax lethal factor (LF) to visualize its membrane translocation inside live cells. In contrast to the previously reported FP tags that significantly perturbed LF's membrane trafficking, our precisely and quantitatively labeled LF exhibited an endocytic activity comparable to wild-type LF. This allowed time-lapse imaging of LF's natural translocation process from host cell membrane to cytosol, which revealed molecular details of its virulence mechanism. Our strategy is generally applicable for monitoring intracellular protein membrane translocation that is difficult to access using conventional protein labeling methodologies.
Language英语
WOS IDWOS:000337095900023
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/167305
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen, Peng R.
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Synthet & Funct Biomol Ctr,Coll Chem, Beijing 100871, Peoples R China
2.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Siqi,Zhang, Gong,Li, Jie,et al. Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2014,53(25):6449-6453.
APA Zheng, Siqi,Zhang, Gong,Li, Jie,&Chen, Peng R..(2014).Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,53(25),6449-6453.
MLA Zheng, Siqi,et al."Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53.25(2014):6449-6453.
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