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A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography
Cai, Jianfeng1; Cheng, Lingping1; Zhao, Jianchao1; Fu, Qing1; Jin, Yu1; Ke, Yanxiong1; Liang, Xinmiao1,2
KeywordHydrophilic Interaction Liquid Chromatography Polyacrylamide Carbohydrates Alcohols
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2017-11-17
ISSN0021-9673
DOI10.1016/j.chroma.2017.10.005
Volume1524Pages:153-159
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLIGHT-SCATTERING DETECTION ; ENE CLICK CHEMISTRY ; VAR. YUNNANENSIS ; POLAR COMPOUNDS ; SAPONINS ; COLUMNS ; UHPLC ; ACID ; HPLC
AbstractA hydrophilic interaction liquid chromatography (HILIC) stationary phase was prepared by a two-step synthesis method, immobilizing polyacrylamide on silica sphere particles. The stationary phase (named PA, 5 mu m dia) was evaluated using a mixture of carbohydrates in HILIC mode and the column efficiency reached 121,000 N m(-1). The retention behavior of carbohydrates on PA stationary phase was investigated with three different organic solvents (acetonitrile, ethanol and methanol) employed as the weak eluent. The strongest hydrophilicity of PA stationary phase was observed in both acetonitrile and methanol as the weak eluent, when compared with another two amide stationary phases. Attributing to its high hydrophilicity, three oligosaccharides (xylooligosaccharide, fructooligosaccharide and chitooligosaccharides) presented good retention on PA stationary phase using alcohols/water as mobile phase. Finally, PA stationary phase was successfully applied for the purification of galactooligosaccharides and saponins of Paris polyphylla. It is feasible to use safer and cheaper alcohols to replace acetonitrile as the weak eluent for green analysis and purification of polar compounds on PA stationary phase. (C) 2017 Published by Elsevier B.V.
Language英语
WOS IDWOS:000415772100016
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168327
Collection中国科学院大连化学物理研究所
Corresponding AuthorJin, Yu; Ke, Yanxiong
Affiliation1.East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China
Recommended Citation
GB/T 7714
Cai, Jianfeng,Cheng, Lingping,Zhao, Jianchao,et al. A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1524:153-159.
APA Cai, Jianfeng.,Cheng, Lingping.,Zhao, Jianchao.,Fu, Qing.,Jin, Yu.,...&Liang, Xinmiao.(2017).A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1524,153-159.
MLA Cai, Jianfeng,et al."A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1524(2017):153-159.
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