DICP OpenIR
Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification
Zhang, Shen1,2; Yuan, Huiming1; Zhao, Baofeng1; Zhang, Lihua1; Zhang, Yukui1
KeywordIntegrated Platform Isotope Dimethyl Labeling Immobilized Enzyme Reactor Dlow Miss-cleavage Digestion
Source PublicationANALYTICA CHIMICA ACTA
2018-02-13
ISSN0003-2670
DOI10.1016/j.aca.2017.10.023
Volume1000Pages:172-179
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; QUANTITATION ; EXPRESSION ; MS/MS
AbstractIn recent years, various enzymatic microreactors and on-line enzyme digestion strategies have been widely applied in high throughput proteome analysis. However, the incomplete and irreproducible digestion would introduce some unexpected variations in comparative proteome quantification when the samples are digested and then chemically isotope labeled in different aliquots. To address these problems, we developed an integrated platform for high throughput proteome quantification with combination of on-line low miss-cleavage protein digestion by an ultra-performance immobilized enzymatic reactor, on-line dimethyl labeling onto a C18 precolumn, peptide separation by two-dimensional nano liquid chromatography and MS detection. Compared to traditional off-line method, such a platform exhibits obvious advantages such as high sensitivity, throughput, accuracy, precision and ease of automation. All these results demonstrated that such a platform might become a promising technique for the quantitative proteome analysis. (c) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000418832900016
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168389
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Shen,Yuan, Huiming,Zhao, Baofeng,et al. Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification[J]. ANALYTICA CHIMICA ACTA,2018,1000:172-179.
APA Zhang, Shen,Yuan, Huiming,Zhao, Baofeng,Zhang, Lihua,&Zhang, Yukui.(2018).Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification.ANALYTICA CHIMICA ACTA,1000,172-179.
MLA Zhang, Shen,et al."Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification".ANALYTICA CHIMICA ACTA 1000(2018):172-179.
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