DICP OpenIR
Proteomics Investigations into Serum Proteins Adsorbed by High-Flux and Low-Flux Dialysis Membranes
Han, Shuai2; Yang, Kaiguang1; Sun, Jingdi2; Liu, Jianhui1; Zhang, Lihua1; Zhao, Jiuyang2
KeywordAdsorbed Proteins Hemodialysis Membrane Proteomics
Source PublicationPROTEOMICS CLINICAL APPLICATIONS
2017-12-01
ISSN1862-8346
DOI10.1002/prca.201700079
Volume11Issue:11-12
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordTANDEM MASS-SPECTROMETRY ; HEMODIALYSIS MEMBRANES ; ATTACK COMPLEX ; ADSORPTION ; DISEASE ; REUSE ; POLYSULFONE ; CLEARANCES ; LECTIN
AbstractHemodialysis is one of the most important therapies for patients with uremia, and the dialysis membrane is the predominant factor that impacts the efficiency of dialysis. Here, a protein adsorption on two different membranes is investigated to provide a basis for improving dialysis materials. Two cases treated with the Polyflux 14L low-flux dialyzer and the Polyflux 140H high-flux dialyzers during two continuous therapies are selected. Four used dialyzers from selected patients are infused with C12Im-Cl to elute the adsorbed proteins. Then labeled digested proteins adsorb by Polyflux 140H and Polyflux 14L with (CD2O)-C-13 and NaCNBD3 (light labeling, L) and CD2O and NaCNBH3 (heavy labeling, H), respectively. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to identify the proteins. According to the ratio (Light labeling/Heavy labeling), the eluted proteins are divided into three groups: significantly higher, significantly lower, and no significant differences with a ratio of > 2, < 0.5, and 0.5-2, respectively. A total of 668 proteins are identified by LC-MS/MS, among which 177 proteins are retained more by the Polyflux 140H membrane (ratio > 2), 320 proteins are retained more by the Polyflux 14L membrane (ratio < 0.5), and 171 proteins show no significant difference (ratio 0.5-2) between the two types of membranes. Statistical significance is shown in the percentage of adsorbed proteins with an isoelectric point (pI) ranging from 9 to 10 (19.08 versus 7.69%; chi(2) = 11.87, p = 0.0006). Proteins with a molecular weight (MW) of 10-15 kDa tend to deposit on Polyflux 140H compared with Polyflux 14L (25 versus 9.23%; chi(2) = 18.66, p = 0.0000) and proteins with a MW of 30-60 kDa tend to deposit on Polyflux 14L compared with Polyflux 140H (36.54 versus 22.37%; chi(2) = 8.96, p = 0.0028). According to gene ontology analysis, the proteins adsorbed by dialysis membranes are closely related to activation of complement system and the coagulation cascade. The proteins adsorbed by Polyflux 140H and Polyflux 14L show significant differences in PI, MW, and protein class. Proteomic techniques are an effective approach for studying hemodialysis membranes.
Language英语
WOS IDWOS:000418253600007
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168443
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua; Zhao, Jiuyang
Affiliation1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Han, Shuai,Yang, Kaiguang,Sun, Jingdi,et al. Proteomics Investigations into Serum Proteins Adsorbed by High-Flux and Low-Flux Dialysis Membranes[J]. PROTEOMICS CLINICAL APPLICATIONS,2017,11(11-12).
APA Han, Shuai,Yang, Kaiguang,Sun, Jingdi,Liu, Jianhui,Zhang, Lihua,&Zhao, Jiuyang.(2017).Proteomics Investigations into Serum Proteins Adsorbed by High-Flux and Low-Flux Dialysis Membranes.PROTEOMICS CLINICAL APPLICATIONS,11(11-12).
MLA Han, Shuai,et al."Proteomics Investigations into Serum Proteins Adsorbed by High-Flux and Low-Flux Dialysis Membranes".PROTEOMICS CLINICAL APPLICATIONS 11.11-12(2017).
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