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Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer
Zheng Si-Jia1,2; Wang Qing-Qing1,2; Wang Xiao-Lin1; Zhao Xin-Jie1; Xu Guo-Wang1
KeywordZwitterionic Hydrophilic Interaction Liquid Chromatography/mass Spectrometry Metabolomics Bladder Cancer Polar Metabolites
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2017-12-01
ISSN0253-3820
DOI10.11895/j.issn.0253.3820.171286
Volume45Issue:12Pages:1921-1929
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPLASMA ; URINE
AbstractBladder cancer (BC) is a fatal malignancy with considerable mortality, and can cause a serious threat to human health. The successful treatment of bladder cancer relies mainly on early detection. Biomarkers are vital to early diagnosis of bladder cancer, and metabonomics play an important role in biomarkers finding. In this study, we used 69 polar metabolites to select the appropriate separation system and develop the zwitterionic hydrophilic chromatography/mass spectrometry (ZIC-HILIC/MS) method. In this method, 50 representative compounds had broad linear ranges between 2-6 orders of magnitude. Moreover the limit of detection of the method was below ng/mL levels. The analysis for six serum samples prepared in parallel showed that this method had good reproducibility, and the RSDs of more than 85% metabolites were less than 30%. Based on this method, it was found that 35 metabolites had significant differences in BC group and healthy control. After screening and validation, the combination of chenodeoxycholic acid, eicosenoic acid, GPC, dodecenoic acid and cystine was a potential biomarker to distinguish BC and normal group. These results indicated that the ZIC. HILIC/MS method could detect diverse metabolites for metabolomic analysis purpose with good reproducibility and stability.
Language英语
WOS IDWOS:000419147100013
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168483
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao Xin-Jie
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zheng Si-Jia,Wang Qing-Qing,Wang Xiao-Lin,et al. Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(12):1921-1929.
APA Zheng Si-Jia,Wang Qing-Qing,Wang Xiao-Lin,Zhao Xin-Jie,&Xu Guo-Wang.(2017).Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(12),1921-1929.
MLA Zheng Si-Jia,et al."Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.12(2017):1921-1929.
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