DICP OpenIR
Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
Wang, Yaqing1,3,4; Wang, Li1; Guo, Yaqiong1,3; Zhu, Yujuan1,3; Qin, Jianhua1,2,3
Source PublicationRSC ADVANCES
2018
ISSN2046-2069
DOI10.1039/c7ra11714k
Volume8Issue:3Pages:1677-1685
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordCEREBROSPINAL-FLUID ; CEREBRAL ORGANOIDS ; INTERSTITIAL FLOW ; HUMAN LIVER ; DISEASE ; TISSUES ; GENERATION ; NEOCORTEX ; CULTURE ; UNIQUE
AbstractBrain organoids derived from the self-organization of human induced pluripotent stem cells (hiPSCs) represent a new class of in vitro organ system for modeling brain development and diseases. However, engineering brain organoids in a biomimetic environment that is favorable for brain development remains challenging. In this work, we present a new strategy to generate hiPSCs-derived 3D brain organoids using an organ-on-a-chip system in a controlled manner. This system provides a biomimetic brain microenvironment by incorporating three-dimensional (3D) Matrigel, fluid flow and multicellular architectures of tissues that allows for extended 3D culture, in situ neural differentiation, and organization of brain organoids on a single device. The generated brain organoids display well-defined neural differentiation, regionalization and cortical organization under perfused culture conditions, which recapitulate the key features of early human brain development. Moreover, the brain organoids exhibit an enhanced expression of cortical layer markers (TBR1 and CTIP2) under perfused cultures as compared to that under static cultures on a Petri dish, indicating the role of mechanical fluid flow in promoting brain organogenesis. The simple and robust brain organoids-on-a-chip system may open new avenues for various stem cell-based organoids engineering and its application in developmental biology and human disease studies.
Language英语
WOS IDWOS:000419371200060
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168505
Collection中国科学院大连化学物理研究所
Corresponding AuthorQin, Jianhua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yaqing,Wang, Li,Guo, Yaqiong,et al. Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system[J]. RSC ADVANCES,2018,8(3):1677-1685.
APA Wang, Yaqing,Wang, Li,Guo, Yaqiong,Zhu, Yujuan,&Qin, Jianhua.(2018).Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system.RSC ADVANCES,8(3),1677-1685.
MLA Wang, Yaqing,et al."Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system".RSC ADVANCES 8.3(2018):1677-1685.
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