DICP OpenIR
Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping
Zhao, Jieyu1,2; Li, Lili1; Zhao, Yanni1; Zhao, Chunxia1; Chen, Xia3; Liu, Pingping3; Zhou, Huina3; Zhang, Junjie1; Hu, Chunxiu1; Chen, Aiguo4; Liu, Guanshan4; Peng, Xiaojun2; Lu, Xin1; Xu, Guowang1
KeywordTopping Tobacco Leaf Metabolomics Liquid Chromatography-mass Spectrometry Gas Chromatography-mass Spectrometry Capillary Electrophoresis-mass Spectrometry
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2018
ISSN1618-2642
DOI10.1007/s00216-017-0596-z
Volume410Issue:3Pages:839-851
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCHROMATOGRAPHY-MASS SPECTROMETRY ; DIFFERENT GEOGRAPHICAL ORIGINS ; FLUE-CURED TOBACCO ; LIQUID-CHROMATOGRAPHY ; PSEUDOTARGETED METABOLOMICS ; SACCHAROMYCES-CEREVISIAE ; CHLOROPHYLL DEGRADATION ; TRANSGENIC RICE ; HEAT-STRESS ; PLANTS
AbstractAs an important cultivation practice used for flue-cured tobacco, topping affects diverse biological processes in the later stages of development and growth. Some studies have focused on using tobacco genes to reflect the physiological changes caused by topping. However, the complex metabolic shifts in the leaf resulting from topping have not yet been investigated in detail. In this study, a comprehensive metabolic profile of primary, secondary, and lipid metabolism in flue-cured tobacco leaf was generated with use of a multiple platform consisting of gas chromatography-mass spectrometry, capillary electrophoresis-mass spectrometry, and liquid chromatography-mass spectrometry/ultraviolet spectroscopy. A total of 367 metabolites were identified and determined. Both principal component analysis and the number of significantly different metabolites indicated that topping had the greatest influence on the upper leaves. During the early stage of topping, great lipid level variations in the upper leaves were observed, and antioxidant defense metabolites were accumulated. This indicated that the topping activated lipid turnover and the antioxidant defense system. At the mature stage, lower levels of senescence-related metabolites and higher levels of secondary metabolites were found in the topped mature leaves. This implied that topping delayed leaf senescence and promoted secondary metabolite accumulation. This study provides a global view of the metabolic perturbation in response to topping.
Language英语
WOS IDWOS:000422952600014
PublisherSPRINGER HEIDELBERG
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168507
Collection中国科学院大连化学物理研究所
Corresponding AuthorLu, Xin; Xu, Guowang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116023, Liaoning, Peoples R China
3.CNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China
4.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Jieyu,Li, Lili,Zhao, Yanni,et al. Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(3):839-851.
APA Zhao, Jieyu.,Li, Lili.,Zhao, Yanni.,Zhao, Chunxia.,Chen, Xia.,...&Xu, Guowang.(2018).Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(3),839-851.
MLA Zhao, Jieyu,et al."Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.3(2018):839-851.
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