DICP OpenIR
"Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment
Shao, Wenya1,2; Liu, Jianxi1,2; Liang, Yu1; Yang, Kaiguang1; Min, Yi1; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
KeywordHydrophilic Interaction Chromatography Mixed-mode Retention Mechanism Separation Glycopeptide Enrichment Branched Copolymer
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2018
ISSN1618-2642
DOI10.1007/s00216-017-0626-x
Volume410Issue:3Pages:1019-1027
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHYDROPHILIC-INTERACTION CHROMATOGRAPHY ; INTERACTION LIQUID-CHROMATOGRAPHY ; MIXED-MODE CHROMATOGRAPHY ; STATIONARY-PHASE ; LINKED GLYCOSYLATION ; MASS-SPECTROMETRY ; PERFORMANCE ; GLYCOPROTEINS ; COMBINATION ; RETENTION
AbstractThree-dimensional branched copolymer, with N,N'-methylene bisacrylamide as the crosslinker and 3-allyloxy-2-hydroxy-1-propane sulfonic acid sodium salt as the monomer, was grafted from silica particles by thiol-ene click reaction. The obtained hydrophilic material with sulfonic acid groups was successfully applied for chromatography separation and glycopeptide enrichment. The separation mechanism was proven as the mixed mode of hydrophilic interaction and cation-exchange by investigating the effect of various chromatographic factors on the retention of polar analytes. By such mixed-mode chromatography, nucleosides, nucleobases, and acidic compounds were successfully separated. The column efficiency was up to 136,000 theoretical plates m(-1) for cytidine, which was much higher than those of previous reports. Furthermore, benefitting from the large amount of hydrophilic groups provided by the branched copolymer, the material was used for the selective enrichment of glycopeptides. Results demonstrated the great potential of such material for chromatography separation and glycoproteome analysis.
Language英语
WOS IDWOS:000422952600032
PublisherSPRINGER HEIDELBERG
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168533
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Shao, Wenya,Liu, Jianxi,Liang, Yu,et al. "Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(3):1019-1027.
APA Shao, Wenya.,Liu, Jianxi.,Liang, Yu.,Yang, Kaiguang.,Min, Yi.,...&Zhang, Yukui.(2018)."Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(3),1019-1027.
MLA Shao, Wenya,et al.""Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.3(2018):1019-1027.
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