DICP OpenIR
Panax ginseng genome examination for ginsenoside biosynthesis
Xu, Jiang1; Chu, Yang1; Liao, Baosheng1; Xiao, Shuiming1; Yin, Qinggang1; Bai, Rui1; Su, He1,2; Dong, Linlin1; Li, Xiwen1; Qian, Jun1; Zhang, Jingjing1; Zhang, Yujun1; Zhang, Xiaoyan1; Wu, Mingli1; Zhang, Jie1; Li, Guozheng3; Zhang, Lei4; Chang, Zhenzhan5; Zhang, Yuebin6; Jia, Zhengwei7; Liu, Zhixiang1; Afreh, Daniel8; Nahurira, Ruth8; Zhang, Lianjuan1; Cheng, Ruiyang1; Zhu, Yingjie1; Zhu, Guangwei1; Rao, Wei7; Zhou, Chao7; Qiao, Lirui7; Huang, Zhihai2; Cheng, Yung-Chi9; Chen, Shilin1
KeywordPanax Ginseng Ginsenosides Genome Mass Spectrometry Imaging
Source PublicationGIGASCIENCE
2017-10-05
ISSN2047-217X
DOI10.1093/gigascience/gix093
Volume6Issue:11
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordMETABOLICALLY ENGINEERED YEASTS ; MULTIPLE SEQUENCE ALIGNMENT ; UDP-GLYCOSYLTRANSFERASES ; FUNCTIONAL-CHARACTERIZATION ; TRITERPENOID SAPONINS ; PHYLOGENETIC ANALYSIS ; MASS-SPECTROMETRY ; READ ALIGNMENT ; TOOL ; IDENTIFICATION
AbstractGinseng, which contains ginsenosides as bioactive compounds, has been regarded as an important traditional medicine for several millennia. However, the genetic background of ginseng remains poorly understood, partly because of the plant's large and complex genome composition. We report the entire genome sequence of Panax ginseng using next-generation sequencing. The 3.5-Gb nucleotide sequence contains more than 60% repeats and encodes 42 006 predicted genes. Twenty-two transcriptome datasets and mass spectrometry images of ginseng roots were adopted to precisely quantify the functional genes. Thirty-one genes were identified to be involved in the mevalonic acid pathway. Eight of these genes were annotated as 3-hydroxy-3-methylglutaryl-CoA reductases, which displayed diverse structures and expression characteristics. A total of 225 UDP-glycosyltransferases (UGTs) were identified, and these UGTs accounted for one of the largest gene families of ginseng. Tandem repeats contributed to the duplication and divergence of UGTs. Molecular modeling of UGTs in the 71st, 74th, and 94th families revealed a regiospecific conserved motif located at the N-terminus. Molecular docking predicted that this motif captures ginsenoside precursors. The ginseng genome represents a valuable resource for understanding and improving the breeding, cultivation, and synthesis biology of this key herb.
Language英语
WOS IDWOS:000419633500001
PublisherOXFORD UNIV PRESS
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168537
Collection中国科学院大连化学物理研究所
Corresponding AuthorCheng, Yung-Chi; Chen, Shilin
Affiliation1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
2.Guangdong Prov Hosp Chinese Med, Guangzhou 510006, Guangdong, Peoples R China
3.China Acad Chinese Med Sci, Natl Data Ctr Tradit Chinese Med, Beijing 100700, Peoples R China
4.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China
5.Peking Univ, Sch Basic Med Sci, Dept Biophys, Hlth Sci Ctr, Beijing 100191, Peoples R China
6.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
7.Waters Corp Shanghai Sci & Technol Co Ltd, Shanghai 201206, Peoples R China
8.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Physiol & Ecol, Minist Agr, Beijing 100081, Peoples R China
9.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA
Recommended Citation
GB/T 7714
Xu, Jiang,Chu, Yang,Liao, Baosheng,et al. Panax ginseng genome examination for ginsenoside biosynthesis[J]. GIGASCIENCE,2017,6(11).
APA Xu, Jiang.,Chu, Yang.,Liao, Baosheng.,Xiao, Shuiming.,Yin, Qinggang.,...&Chen, Shilin.(2017).Panax ginseng genome examination for ginsenoside biosynthesis.GIGASCIENCE,6(11).
MLA Xu, Jiang,et al."Panax ginseng genome examination for ginsenoside biosynthesis".GIGASCIENCE 6.11(2017).
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