DICP OpenIR
Determination of tropane alkaloids by heart cutting reversed phase - Strong cation exchange two dimensional liquid chromatography
Long, Zhen1; Zhang, Yanhai1; Gamache, Paul2; Guo, Zhimou3; Steiner, Frank4; Du, Nana4; Liu, Xiaoda1; Jin, Yan1; Liu, Xingguo1; Liu, Lvye1
KeywordHeart Cutting Two Dimensional Liquid Chromatography Strong Cation Exchange Reversed Phase Liquid Chromatography Tropane Alkaloid Herbal Medicine
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2018
ISSN1570-0232
DOI10.1016/j.jchromb.2017.10.064
Volume1072Pages:70-77
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordFLIGHT MASS-SPECTROMETRY ; BASIC COMPOUNDS ; STATIONARY PHASES ; QUALITY-CONTROL ; SCX-RP ; SEPARATION ; PURIFICATION ; SCOPOLAMINE ; ENRICHMENT ; RESOLUTION
AbstractCurrent Chinese Pharmacopoeia (ChP) standards apply liquid extraction combined with one dimensional liquid chromatography (1DLC) method for determining alkaloids in herbal medicines. The complex pretreatments lead to a low analytical efficiency and possible component loss. In this study, a heart cutting reversed phase - strong cation exchange two dimensional liquid chromatography (RP - SCX 2DLC) approach was optimized for simultaneously quantifying tropane alkaloids (anisodine, scopolamine and hyoscyamine) in herbal medicines and herbal medicine tablets without further treatment of the filtered extract. The chromatographic conditions were systematically optimized in terms of column type, mobile phase composition and flow rate. To improve peak capacity and obtain symmetric peak shape of alkaloids, a polar group embedded C18 column combined with chaotropic salts was used in the first dimension. To remove the disturbance of non-alkaloids, achieve unique selectivity and acquire symmetric peak shape of alkaloids, an SCX column combined with phosphate buffer was used in the second dimension. Method validation was performed in terms of linearity, precision (0.54-0.82%), recovery (94.1-105.2%), limit of detection (LOD) and limit of quantification (LOQ) of the three analytes varied between 0.067-0.115 mg L-1 and 0.195-0.268 mg L-1, respectively. The method demonstrated superiority over 1DLC method in respect of resolution (less alkaloid co-eluted), sample preparation (no pretreatment procedure) and transfer rate (minimum component loss). The optimized RP - SCX 2DLC approach was subsequently applied to quantify target alkaloids in five herbal medicines and herbal medicine tablets from three different manufactures. The results demonstrated that the developed heart cutting RP - SCX 2DLC approach represented a new, strategically significant methodology for the quality evaluation of tropane alkaloid in related herbal medicines that involve complex chemical matrix.
Language英语
WOS IDWOS:000423637600009
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168609
Collection中国科学院大连化学物理研究所
Corresponding AuthorGuo, Zhimou; Jin, Yan
Affiliation1.Thermofisher Sci Corp, Beijing 100080, Peoples R China
2.ThermoFisher Sci Corp, Chelmsford, MA 01824 USA
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
4.Themofisher Sci Corp, D-82110 Germering, Germany
Recommended Citation
GB/T 7714
Long, Zhen,Zhang, Yanhai,Gamache, Paul,et al. Determination of tropane alkaloids by heart cutting reversed phase - Strong cation exchange two dimensional liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2018,1072:70-77.
APA Long, Zhen.,Zhang, Yanhai.,Gamache, Paul.,Guo, Zhimou.,Steiner, Frank.,...&Liu, Lvye.(2018).Determination of tropane alkaloids by heart cutting reversed phase - Strong cation exchange two dimensional liquid chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1072,70-77.
MLA Long, Zhen,et al."Determination of tropane alkaloids by heart cutting reversed phase - Strong cation exchange two dimensional liquid chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1072(2018):70-77.
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