DICP OpenIR
A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement
Li, Tian1,2; Wu, Xi2; Tao, Guangyu2; Yin, Haoyan3; Zhang, Junlong3; Liu, Feng2; Li, Na2
KeywordGold Nanoparticle Dark-field Imaging Enumeration Nucleic Acid Target-induced Strand-displacement
Source PublicationBIOSENSORS & BIOELECTRONICS
2018-05-15
ISSN0956-5663
DOI10.1016/j.bios.2018.01.034
Volume105Pages:137-142
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordDARK-FIELD MICROSCOPY ; SINGLE PLASMONIC NANOPARTICLES ; NUCLEIC-ACID DETECTION ; LIGHT-SCATTERING ; LIVING CELLS ; ONE-STEP ; PARTICLE LEVEL ; HYBRIDIZATION ; QUANTIFICATION ; NANOMATERIALS
AbstractBy combining the gold nanoparticle (AuNP) enumeration with target-induced strand-displacement reaction, we have developed a non-amplification platform for DNA and miRNA detection based on a deliberately designed sandwich-structured nanocomplex probe (SNC Probe). The proposed strategy can realize the sensitive detection of nucleic acids within 40 min with the detection limit of 6.6 fM for HBV DNA and 13.5 fM for miRNA-141, respectively. The method presents reasonable ability to discriminate miRNA-141 from all the other members of miRNA-200 family. And it can also be used for direct detection of miRNA-141 in samples of extracted total small RNA from different cell lines which are reported to have altered levels of miRNA-141. Furthermore, the spike recovery (n = 3) of miRNA-141 in total small RNA extracts of Hela cells is found to be 92.8% for 20 p.M. and 94.7% for 100 p.M. with the standard deviation of 9.2% and 6.8%, respectively. As the only reagent involved in the assay, the SNC Probe presents a very good stability with a relative standard deviation of 3.3% amongst eight tests in 30 days, which greatly simplifies the assay procedure and presents the suitability for routine analyses. On the basis of these findings, this simple non-amplification assay platform can be expected to find assorted applications that can make the best use of the simplicity and sensitivity.
Language英语
WOS IDWOS:000426024200021
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168673
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Na
Affiliation1.Henan Univ Sci & Technol, Med Coll, Luoyang 471003, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,BNLMS, Beijing 100871, Peoples R China
3.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Li, Tian,Wu, Xi,Tao, Guangyu,et al. A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement[J]. BIOSENSORS & BIOELECTRONICS,2018,105:137-142.
APA Li, Tian.,Wu, Xi.,Tao, Guangyu.,Yin, Haoyan.,Zhang, Junlong.,...&Li, Na.(2018).A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement.BIOSENSORS & BIOELECTRONICS,105,137-142.
MLA Li, Tian,et al."A simple and non-amplification platform for femtomolar DNA and microRNA detection by combining automatic gold nanoparticle enumeration with target-induced strand-displacement".BIOSENSORS & BIOELECTRONICS 105(2018):137-142.
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