DICP OpenIR
Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides
Su, Jie1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
KeywordMagnetic Graphene Oxide Mesoporous Silica Adenosine Triphosphate Titanium Ion Phosphopeptide Enrichment
Source PublicationACS SUSTAINABLE CHEMISTRY & ENGINEERING
2018-02-01
ISSN2168-0485
DOI10.1021/acssuschemeng.7b03607
Volume6Issue:2Pages:2188-2196
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Multidisciplinary ; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY ; Engineering, Chemical
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Engineering
WOS KeywordGRAPHENE/MESOPOROUS SILICA COMPOSITES ; AFFINITY-CHROMATOGRAPHY PLATFORM ; MASS-SPECTROMETRY ANALYSIS ; N-LINKED GLYCAN ; ENDOGENOUS PHOSPHOPEPTIDES ; DESIGNED SYNTHESIS ; PHOSPHOPROTEOME ANALYSIS ; FACILE PREPARATION ; ORGANIC FRAMEWORK ; HYBRID MATERIAL
AbstractDeveloping an efficient strategy to enrich the low abundance phosphopeptides before mass spectrometry detection is a vital preprocessing step in phosphoproteomics. In this work, we synthesized an adenosine phosphate-Ti4+ functionalized magnetic mesoporous graphene oxide nanocomposite (denoted as MG@ mSiO(2)-ATP-Ti4+) to selectively extract phosphorylated peptides from complex biological samples based on the immobilized metal ion affinity chromatography (IMAC). Mesoporous silica was coated on the substrate material of magnetic graphene oxide and then the ATP containing three phosphate groups was grafted on the inwall of mesoporous channels as chelating ligands to immobilize the Ti4+ cations. With favorable properties, such as large surface area and good hydrophilicity and size-exclusion effect, the MG@mSiO(2)-ATP-Ti4+ exhibited excellent sensitivity and selectivity toward phosphopeptides whether in low concentration of beta-casein digest (20 amol mu L-1, 4 fmol) or the digest mixture of beta-casein and bovine serum albumin (with molar ratio of 1:1000) as well as good reusability. Furthermore, MG@mSiO(2)-ATP-Ti4+ could also be applied in the selective enrichment of phosphorylated peptides from nonfat milk digest and human saliva and serum.
Language英语
WOS IDWOS:000424728300073
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168703
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen, Langxing
Affiliation1.Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Su, Jie,He, Xiwen,Chen, Langxing,et al. Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2018,6(2):2188-2196.
APA Su, Jie,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2018).Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,6(2),2188-2196.
MLA Su, Jie,et al."Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 6.2(2018):2188-2196.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Su, Jie]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Su, Jie]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Su, Jie]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.