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SNAP-tag fluorogenic probes for wash free protein labeling
Leng, Shuang1,2; Qiao, Qing-Long2,3; Gao, Yue1; Miao, Lu2; Deng, Wu-Guo1; Xu, Zhao-Chao2
KeywordFluorogenic Probes Wash Free Snap-tag Protein Labeling
Source PublicationCHINESE CHEMICAL LETTERS
2017-10-01
ISSN1001-8417
DOI10.1016/j.cclet.2017.03.034
Volume28Issue:10Pages:1911-1915
Indexed BySCI
SubtypeReview
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordLIVE-CELL ; FLUORESCENT-PROBE ; FUSION PROTEINS ; O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ; DIRECTED EVOLUTION ; LIVING CELLS ; IN-VIVO ; FLUOROPHORE ; TECHNOLOGY ; INDICATORS
AbstractProtein labeling by using a protein tag and tag-specific fluorescent probes is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. SNAP-tag as one of the most prominent fusion tags has been widely used and already commercially available. Recently, various fluorogenic probes for SNAP-tag based protein labeling were reported. Owing to turn-on fluorescence response, fluorogenic probes for SNAP-tag minimize the fluorescence background caused by unreacted or nonspecifically bound probes and allow for direct imaging in living cells without wash-out steps. Thus, real-time analysis of protein localization, dynamics and interactions has been made possible by SNAP-tag fluorogenic probes. In this review, we describe the design strategies of fluorogenic probes for SNAP-tag and their applications in cellular protein labeling. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000426222000001
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168731
Collection中国科学院大连化学物理研究所
Corresponding AuthorMiao, Lu; Deng, Wu-Guo; Xu, Zhao-Chao
Affiliation1.Dalian Med Univ, Inst Canc Stem Cell, Canc Ctr, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Leng, Shuang,Qiao, Qing-Long,Gao, Yue,et al. SNAP-tag fluorogenic probes for wash free protein labeling[J]. CHINESE CHEMICAL LETTERS,2017,28(10):1911-1915.
APA Leng, Shuang,Qiao, Qing-Long,Gao, Yue,Miao, Lu,Deng, Wu-Guo,&Xu, Zhao-Chao.(2017).SNAP-tag fluorogenic probes for wash free protein labeling.CHINESE CHEMICAL LETTERS,28(10),1911-1915.
MLA Leng, Shuang,et al."SNAP-tag fluorogenic probes for wash free protein labeling".CHINESE CHEMICAL LETTERS 28.10(2017):1911-1915.
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