DICP OpenIR
A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins
Su, Jie1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
KeywordMagnetic Graphene Oxide Click Chemistry Si-atrp Boronate Affinity Glycoprotein Enrichment
Source PublicationTALANTA
2018-04-01
ISSN0039-9140
DOI10.1016/j.talanta.2017.12.037
Volume180Pages:54-60
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSELECTIVE ENRICHMENT ; EFFICIENT ENRICHMENT ; MONOLITHIC COLUMN ; HIGH-CAPACITY ; IONIC LIQUID ; NANOPARTICLES ; CAPTURE ; RECOGNITION ; FACILE ; GLYCOPEPTIDES
AbstractAn efficient glycoproteins enrichment platform is one of vital preprocessing steps in biomarker research and in particular glycoproteomics. In this work, a well-defined boronic acid functionalized magnetic graphene oxide nanocomposite (Fe3O4-GO@PAAPBA) was synthesized for the selective enrichment of glycoproteins from complex biological samples via a novel strategy based on the thiol-ene click chemistry and surface initiated atom transfer radical polymerization (SI-ATRP). The initiator of ATRP was anchored to the surface of substrate through thiol-ene click reaction. The product Fe3O4-GO@PAAPBA was successfully synthesized in following SI-ATRP. The Fe3O4-GO@PAAPBA nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM) and thermogravimetric analysis. The adsorption capacity of Fe3O4-GO@PAAPBA towards ovalbumin (OVA) and transferrin (Trf) is 471 mg g(-1) and 450 mg g(-1), respectively. The nanocomposite also featured good selectivity to glycoproteins in the mixture of glycoproteins and non-glycoproteins at alkaline (pH 9.0) and physiological conditions (pH 7.4). Furthermore, it can be applied to extract glycoproteins directly from egg white samples. These results have indicated that Fe3O4-GO@PAAPBA was a potential affinity material in glycoprotein analysis.
Language英语
WOS IDWOS:000424717900008
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168797
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen, Langxing
Affiliation1.Nankai Univ, Res Ctr Analyt Sci, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit,State K, Tianjin 300071, Peoples R China
2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Su, Jie,He, Xiwen,Chen, Langxing,et al. A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins[J]. TALANTA,2018,180:54-60.
APA Su, Jie,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2018).A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins.TALANTA,180,54-60.
MLA Su, Jie,et al."A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins".TALANTA 180(2018):54-60.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Su, Jie]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Su, Jie]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Su, Jie]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.