DICP OpenIR
Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast
Zhou, Yongjin J.1,2; Hu, Yating2,3; Zhu, Zhiwei2,3; Siewers, Verena2,3; Nielsen, Jens2,3,4
KeywordYeast Cell Factories Fatty Acids Hydrocarbons Transporter Metabolic Engineering
Source PublicationACS SYNTHETIC BIOLOGY
2018-02-01
ISSN2161-5063
DOI10.1021/acssynbio.7b00338
Volume7Issue:2Pages:584-590
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordSACCHAROMYCES-CEREVISIAE ; ESCHERICHIA-COLI ; FATTY-ACIDS ; RHODOSPORIDIUM-TORULOIDES ; YARROWIA-LIPOLYTICA ; EXTRACTION ; EXPRESSION ; PATHWAY ; CULTURE ; OLEOCHEMICALS
AbstractMicrobial production of fatty acid-derived hydrocarbons offers a great opportunity to sustainably supply biofuels and oleochemicals. One challenge is to achieve a high production rate. Besides, low efficiency in secretion will cause high separation costs, and it is therefore desirable to have product secretion. Here, we engineered the budding yeast Saccharomyces cerevisiae to produce and secrete 1-alkenes by manipulation of the fatty acid metabolism, enzyme selection, engineering the electron transfer system and expressing a transporter. Furthermore, we implemented a dynamic regulation strategy to control the expression of membrane enzyme and 1-alkene production and cell growth by relieving the possible toxicity of overexpressed membrane proteins. With these efforts, the engineered yeast cell factory produced 35.3 mg/L 1-alkenes with more than 80% being secreted. This represents a 10-fold improvement compared with earlier reported hydrocarbon production by S. cerevisiae.
Language英语
WOS IDWOS:000426012600029
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168799
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhou, Yongjin J.; Nielsen, Jens
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Chalmers Univ Technol, Dept Biol & Biol Engn, Kemivagen 10, SE-41296 Gothenburg, Sweden
3.Chalmers Univ Technol, Ctr Biosustainabil, Novo Nordisk Fdn, SE-41296 Gothenburg, Sweden
4.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark
Recommended Citation
GB/T 7714
Zhou, Yongjin J.,Hu, Yating,Zhu, Zhiwei,et al. Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast[J]. ACS SYNTHETIC BIOLOGY,2018,7(2):584-590.
APA Zhou, Yongjin J.,Hu, Yating,Zhu, Zhiwei,Siewers, Verena,&Nielsen, Jens.(2018).Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast.ACS SYNTHETIC BIOLOGY,7(2),584-590.
MLA Zhou, Yongjin J.,et al."Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast".ACS SYNTHETIC BIOLOGY 7.2(2018):584-590.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou, Yongjin J.]'s Articles
[Hu, Yating]'s Articles
[Zhu, Zhiwei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, Yongjin J.]'s Articles
[Hu, Yating]'s Articles
[Zhu, Zhiwei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, Yongjin J.]'s Articles
[Hu, Yating]'s Articles
[Zhu, Zhiwei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.