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3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions
Wu, Qiong1,2; Jiang, Bo1; Weng, Yejing1,2; Liu, Jianxi1,3; Li, Senwu1,2; Hu, Yechen1,2; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
Source PublicationANALYTICAL CHEMISTRY
2018-02-20
ISSN0003-2700
DOI10.1021/acs.analchem.7b04451
Volume90Issue:4Pages:2671-2677
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordDISTILLATION-PRECIPITATION POLYMERIZATION ; SELECTIVE ENRICHMENT ; BORONATE AFFINITY ; MONOLITHIC CAPILLARY ; TRACE GLYCOPROTEINS ; CIS-DIOL ; PH 7.4 ; NANOPARTICLES ; BINDING ; RECOGNITION
AbstractBoronate affinity materials have been successfully used for the selective recognition of glycoproteins. However, by such materials, the large-scale glycoproteins enrichment from human plasma under physiological conditions is rarely reported. In this work, 3-carboxybenzoboroxole (CBX) functionalized polyethylenimine (PEI) modified magnetic graphene oxide nanocomposites were synthesized. Benefitting from the low pK(a) value of CBX (similar to 6.9) and PEI dendrimer-assisted multivalent binding, the Freundlich constant (K-F) for the adsorption of horseradish peroxidase (HRP) was 3.0-7.3 times higher than that obtained by previous work, displaying the high enrichment capacity. Moreover, PEI could improve the hydrophilicity of nanocomposites and reduce nonglycoprotein adsorption. Therefore, such nanocomposites were successfully applied to the analysis of human plasma glycoproteome under physiological conditions, and the identified glycoproteins number and recognition selectivity was increased when compared to the results obtained by previous boronic acid-functionalized particles (Sil@Poly(APBA-co-MBAAm)) under common alkaline condition (137 vs 78 and 67.8% vs 57.8%, respectively). In addition, thrombin (F2), an important plasma glycoprotein, labile under alkaline conditions, was specifically identified by our method, demonstrating the great promise of such nanocomposites in the deep-coverage glycoproteome analysis.
Language英语
WOS IDWOS:000426143100040
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168821
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Wu, Qiong,Jiang, Bo,Weng, Yejing,et al. 3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions[J]. ANALYTICAL CHEMISTRY,2018,90(4):2671-2677.
APA Wu, Qiong.,Jiang, Bo.,Weng, Yejing.,Liu, Jianxi.,Li, Senwu.,...&Zhang, Yukui.(2018).3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions.ANALYTICAL CHEMISTRY,90(4),2671-2677.
MLA Wu, Qiong,et al."3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions".ANALYTICAL CHEMISTRY 90.4(2018):2671-2677.
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