DICP OpenIR
High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine
Wang, Yan1,2; Hua, Lei1; Jiang, Jichun1; Xie, Yuanyuan1; Hou, Keyong1; Li, Qingyun3; Wu, Chenxin1,2; Li, Haiyang1
KeywordHigh-pressure Photon Ionization Mass Spectrometry Dynamic Purge-injection Urine Metabolomics 2 5-dimethylpyrrole
Source PublicationANALYTICA CHIMICA ACTA
2018-05-30
ISSN0003-2670
DOI10.1016/j.aca.2018.01.006
Volume1008Pages:74-81
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSOLID-PHASE MICROEXTRACTION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRIMETHYLAMINE-N-OXIDE ; GAS-CHROMATOGRAPHY ; ORGANIC-COMPOUNDS ; CANCER BIOMARKERS ; SOIL SAMPLES ; HS-SPME ; HEADSPACE ; METABOLOMICS
AbstractSmall molecule metabolites are widely used as biomarkers in the research field of metabolomics for disease diagnosis and exposure assessment. As a readily available biofluid containing plenty of volatile organic metabolites (VOMs), urine is ideal for non-invasive metabolomic analysis; however, there is still lack of rapid analysis method for VOMs in urine. Here we report a kind of rapid method for urine analysis by employing high-pressure photon ionization time-of-flight mass spectrometry (HPPI-TOFMS) combined with dynamic purge-injection. Various types of metabolites, such as ketones, alcohols, acids, sulfides, pyrroles and amines were detected directly by simple acidification or alkalization of urines. It is noteworthy that nitrogen-containing compounds, especially polar amines, could be ultrasensitively measured without any derivatization. The analytical capability of the direct HPPI-MS technique was demonstrated by analyzing five valuable metabolites, i.e., toluene, 2,5-dimethylpyrrole, trimethlyamine, styrene, and p-xylene, which exhibited relatively low limits of detection, wide linear range and satisfactory repeatability. Being highly sensitive and humidity-friendly, the whole analytical procedure is easily operated in less than 6 min. Interestingly, a new biomarker 2,5-dimethylpyrrole was exclusively found in the smoker's urine sample besides toluene. The work presents a novel tool for rapid nontarget disease biomarkers screening or target monitoring of specific compounds through the investigation of volatile metabolites in urine. (C) 2018 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000424539800009
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168837
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Haiyang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100039, Peoples R China
3.Jilin Univ, Dept Instrumentat & Elect Engn, Changchun 130021, Jilin, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yan,Hua, Lei,Jiang, Jichun,et al. High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine[J]. ANALYTICA CHIMICA ACTA,2018,1008:74-81.
APA Wang, Yan.,Hua, Lei.,Jiang, Jichun.,Xie, Yuanyuan.,Hou, Keyong.,...&Li, Haiyang.(2018).High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine.ANALYTICA CHIMICA ACTA,1008,74-81.
MLA Wang, Yan,et al."High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine".ANALYTICA CHIMICA ACTA 1008(2018):74-81.
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