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Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography
Ma, Shujuan1,2; Zhang, Haiyang1,2; Li, Ya1,2; Li, Yanan2,3; Zhang, Na2,3; Ou, Junjie2; Ye, Mingliang2; Wei, Yinmao1
KeywordHybrid Monoliths Thiol-yne Click Reaction Photo-initiated Polymerization Capillary Liquid Chromatography 1 1 3-diethynyltetramethyl-disiloxane 6-hexanedithiol
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2018-02-23
ISSN0021-9673
DOI10.1016/j.chroma.2018.01.028
Volume1538Pages:8-16
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordENE CLICK CHEMISTRY ; SMALL MOLECULES ; ACRYLATE POLYMERIZATION ; FACILE PREPARATION ; PERFORMANCE ; SEPARATION ; EFFICIENCY
AbstractAlthough several approaches have been developed to fabricate hybrid monoliths, it would still take a few hours to finish the formation of monoliths. Herein, photo-initiated thiol-yne polymerization was first adopted to in situ fabricate hybrid monoliths within the confines of UV-transparent fused-silica capillary. A silicon-containing diyne (1,3-diethynyltetramethyl-disiloxane, DYDS) was copolymerized with three multithiols, 1,6-hexanedithiol, trimethylolpropane tris(3-mercaptopropionate) and pentaerythriol tetrakis(3-mercaptopropionate), by using a binary porogenic system of diethylene glycol diethyl ether (DEGDE)/poly(ethylene glycol) (PEG200) within 10 min. Several characterizations of three hybrid monoliths (assigned as I, II and III, respectively) were performed. The results showed that these hybrid monoliths possessed bicontinuous porous structure, which was remarkably different from that via typical free-radical polymerization. The highest column efficiency of 76,000 plates per meter for butylbenzene was obtained on the column I in reversed-phase liquid chromatography (RPLC). It was observed that the efficiencies for strong-retained butylbenzene were almost close to those of weak-retained benzene, indicating a retention-independent efficient performance of small molecules on hybrid column I. The surface area of this hybrid monolith was very small in the dry state (less than 10.0 m(2)/g), and the chromatographic behavior of hybrid monolithic columns would be possibly explained by radical-mediated step-growth process of thiol-yne polymerization. Finally, the column I was applied for separation of BSA tryptic digest by cLC-MS/MS, indicating satisfactory separation ability for complicated samples. (C) 2018 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000426230700002
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168887
Collection中国科学院大连化学物理研究所
Corresponding AuthorWei, Yinmao
Affiliation1.Northwest Univ, Minist Educ, Coll Chem & Mat Sci, Key Lab Synth & Nat Funct Mol Chem, Xian 710069, Shaanxi, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Ma, Shujuan,Zhang, Haiyang,Li, Ya,et al. Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1538:8-16.
APA Ma, Shujuan.,Zhang, Haiyang.,Li, Ya.,Li, Yanan.,Zhang, Na.,...&Wei, Yinmao.(2018).Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1538,8-16.
MLA Ma, Shujuan,et al."Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1538(2018):8-16.
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