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Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks
Peng, Jinlin1; Zhao, Yaopeng4; Hong, Yulong1; Burkhalter, Robert S.2; Hogue, Carrie L.2; Tran, Elizabeth1; Wei, Lai4; Romeo, Lori3; Dolley-Sonneville, Paula3; Melkoumian, Zara3; Liang, Xinmiao4; Fang, Ye1
Source PublicationANALYTICAL CHEMISTRY
2018-04-03
ISSN0003-2700
DOI10.1021/acs.analchem.7b05102
Volume90Issue:7Pages:4603-4610
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordBISPHENOL-A POLYCARBONATE ; CONTAMINANTS LEACH ; DRUG DISCOVERY ; GPR35 AGONISTS ; PLASTIC TUBES ; CHO-CELLS ; GENOME ; IMPACT ; 3,3'-DINITROBISPHENOL ; IDENTIFICATION
AbstractThis paper reports the chemical identity and mechanism of action and formation of a cell growth inhibitory compound leached from some single-use Erlenmeyer polycarbonate shaker flasks under routine cell culture conditions. Single-use cell culture vessels have been increasingly used for the production of biopharmaceuticals; however, they often suffer from issues associated with leachables that may interfere with cell growth and protein stability. Here, high-performance liquid-chromatography preparations and cell proliferation assays led to identification of a compound from the water extracts of some polycarbonate flasks, which exhibited subline- and seeding density-dependent growth inhibition of CHO cells in suspension culture. Mass spectroscopy, nuclear magnetic resonance spectroscopy, and chemical synthesis confirmed that this compound is 3,S-dinitro-bisphenol A. Cell cycle analysis suggests that 3,5-dinitro-bisphenol A arrests CHO-S cells at the G(1)/G(0) phase. Dynamic mass redistribution assays showed that 3,5-dinitro-bisphenol A is a weak GPR3S agonist. Analysis of the flask manufacturing process suggests that 3,5-dinitro-bisphenol A is formed via the combination of molding process with gamma-sterilization. This is the first report of a cell culture/assay interfering leachable compound that is formed through gamma-irradiation-mediated nitric oxide free radical reaction.
Language英语
WOS IDWOS:000429385800051
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/168985
Collection中国科学院大连化学物理研究所
Corresponding AuthorMelkoumian, Zara; Liang, Xinmiao; Fang, Ye
Affiliation1.Corning Inc, Corning R&D Corp, Biochem Technol, Corning, NY 14831 USA
2.Corning Inc, Corning R&D Corp, Characterizat Sci, Corning, NY 14831 USA
3.Corning Inc, Corning R&D Corp, Corning Life Sci Dev, Corning, NY 14831 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Peng, Jinlin,Zhao, Yaopeng,Hong, Yulong,et al. Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks[J]. ANALYTICAL CHEMISTRY,2018,90(7):4603-4610.
APA Peng, Jinlin.,Zhao, Yaopeng.,Hong, Yulong.,Burkhalter, Robert S..,Hogue, Carrie L..,...&Fang, Ye.(2018).Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks.ANALYTICAL CHEMISTRY,90(7),4603-4610.
MLA Peng, Jinlin,et al."Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks".ANALYTICAL CHEMISTRY 90.7(2018):4603-4610.
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