DICP OpenIR
A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems
Geng, Xuhui1; Shi, Meng1,2; Ning, Haijing1; Feng, Chunbo1; Guan, Yafeng1
KeywordLaser Induced Fluorescence Compact Low-cost Single Cell
Source PublicationTALANTA
2018-05-15
ISSN0039-9140
DOI10.1016/j.talanta.2018.01.076
Volume182Pages:279-284
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordLIGHT-EMITTING-DIODES ; SINGLE-CELL ; LIQUID-CHROMATOGRAPHY ; ELECTROPHORESIS
AbstractA compact and low-cost laser induced fluorescence (LIF) detector based on confocal structure for capillary flow systems was developed and applied for analysis of Her2 protein on single Hela cells. A low-power and low-cost 450 nm laser diode (LD) instead of a high quality laser was used as excitation light source. A compact optical design together with shortened optical path length improved the optical efficiency and detection sensitivity. A superior silicon based photodetector assembly was used for fluorescence detection instead of a photomultiplier (PMT). The limit of detection (LOD) for fluorescein sodium was 3 x 10(-12) M or 165 fluorescein molecules in detection volume measured on a homemade capillary electroosmotic driven (EOD)-LIF system, which was similar to commercial LIFs. Compared to commercial LIFs, the whole volume of our LIF was reduced to 1/2-1/3, and the cost was less than 1/3 of them.
Language英语
WOS IDWOS:000428229200035
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169015
Collection中国科学院大连化学物理研究所
Corresponding AuthorGuan, Yafeng
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Geng, Xuhui,Shi, Meng,Ning, Haijing,et al. A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems[J]. TALANTA,2018,182:279-284.
APA Geng, Xuhui,Shi, Meng,Ning, Haijing,Feng, Chunbo,&Guan, Yafeng.(2018).A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems.TALANTA,182,279-284.
MLA Geng, Xuhui,et al."A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems".TALANTA 182(2018):279-284.
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