DICP OpenIR
Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells
Sun, Dongsheng1,2,3; Liu, Yang2; Wu, Hao2,3; Ren, Ying3,4; Ma, Xiaojun4; Wu, Huijian1; Sun, Guangwei2
KeywordAlginate Gel Bead Gelling Bath Mass Transfer Proliferation Three-dimensional Culture
Source PublicationBIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
2018-03-01
ISSN0885-4513
DOI10.1002/bab.1585
Volume65Issue:2Pages:263-273
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordBIOARTIFICIAL LIVER ; IMMOBILIZATION MATERIAL ; SPHEROID CULTURE ; C3A CELLS ; MICROCAPSULES ; MATRIX ; MODEL ; HEPATOCYTES ; SUPPORT ; POLYCATION
AbstractOptimizing alginate gel beads is necessary to support the survival, proliferation, and function of entrapped hepatocytes. In this study, gelling bath was modified by decreasing calcium ion concentration and increasing sodium ion concentration. Alginate gel beads (using 36% G sodium alginate) prepared in the modified gelling bath had more homogeneous structure and better mass transfer properties compared with the traditional gelling bath that contains only calcium ions. Moreover, alginate gel beads generated in the modified gelling bath could significantly promote the HepG2 cell proliferation and the growth of cell spheroids, and maintain the albumin secretion ability similar to alginate gel beads prepared in the traditional gelling bath with only calcium ions. The mass transfer properties and cell proliferation were similar in ALG beads with different M/G ratio (36% G and 55% G) generated in the modified gelling bath, whereas they were significantly increased compared with alginate gel beads (55% G) in traditional gelling bath. These results indicated that adjusting the gelling bath was a simple and convenient method to enhance the mass transfer properties of alginate gel beads for 3D hepatocyte culture, which might provide more hepatocytes for the bioartificial liver support system.
Language英语
WOS IDWOS:000430115900019
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169037
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yang; Wu, Huijian; Sun, Guangwei
Affiliation1.Dalian Univ Technol, Sch Life Sci & Biotechnol, 2 Ling Gong Rd, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Sun, Dongsheng,Liu, Yang,Wu, Hao,et al. Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2018,65(2):263-273.
APA Sun, Dongsheng.,Liu, Yang.,Wu, Hao.,Ren, Ying.,Ma, Xiaojun.,...&Sun, Guangwei.(2018).Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,65(2),263-273.
MLA Sun, Dongsheng,et al."Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 65.2(2018):263-273.
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