DICP OpenIR
Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells
Sun, Dongsheng1,2,3; Liu, Yang1; Wang, Huihui1,2; Deng, Fei2,4; Zhang, Ying1; Zhao, Shan1; Ma, Xiaojun4; Wu, Huijian3; Sun, Guangwei1
KeywordHepatocellular Carcinoma Three-dimensional (3d) Cell Culture Decellularized Liver Matrix Alginate Gel Bead Urokinase Plasminogen Activator Matrix Metalloproteinase
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2018-04-01
ISSN0141-8130
DOI10.1016/j.ijbiomac.2017.11.103
Volume109Pages:1154-1163
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS KeywordPLASMINOGEN ACTIVATION SYSTEM ; EXTRACELLULAR-MATRIX ; COLORECTAL-CANCER ; IN-VITRO ; TUMOR MICROENVIRONMENT ; 3-DIMENSIONAL CULTURE ; SCAFFOLD MATERIAL ; TISSUE ; METALLOPROTEINASES ; EXPRESSION
AbstractDeveloping reliable three-dimensional (3D) cell culture systems that can mimic native tumor microenvironments is necessary for investigating the mechanism of hepatocellular carcinoma (HCC) metastasis and screen therapeutic drugs. In the present study, we developed decellularized liver matrix-alginate (DLM-ALG) hybrid gel beads. DLM powder was prepared by optimized decellularization methods and liquid nitrogen grinding. DLM-ALG beads were generated by dropping alginate solution containing DLM powder into a gelling bath. DLM powder concentration in alginate solution was <= 1% (w/v) and had no effect on the sphericity and mechanical stability of the beads. In addition, HCCLM3 cells cultured in 1% (w/v) DLM-ALG beads presented gradually enhanced viability during in vitro culture. The protein expression of urokinase plasminogen activator system and activity of matrix metalloproteinases (MMPs) of HCCLM3 cells, including MMP2 and MMP9, were more significantly promoted in DLM-ALG beads compared with that in conventional ALG beads without DLM powder. Moreover, the dose-dependent increase in HCCLM3 cell MMP activities was observed along with the DLM powder concentration in 0.5% and 1% DLM-ALG groups. Therefore, DLM-ALG beads might serve as a novel 3D culture system for exploring the mechanisms of HCC metastasis and screening therapeutic drugs. (c) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000428099900125
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169047
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yang; Sun, Guangwei
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
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Sun, Dongsheng,Liu, Yang,Wang, Huihui,et al. Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,109:1154-1163.
APA Sun, Dongsheng.,Liu, Yang.,Wang, Huihui.,Deng, Fei.,Zhang, Ying.,...&Sun, Guangwei.(2018).Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,109,1154-1163.
MLA Sun, Dongsheng,et al."Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 109(2018):1154-1163.
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