DICP OpenIR
Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans
Li, Xin1,2; Xu, Guiju1,2; Peng, Jiaxi1,2; Liu, Shengju1,2; Zhang, Hongyan1,2; Mao, Jiawei1,2; Niu, Huan1,2; Lv, Wenping1; Zhao, Xingyun1,2; Wu, Ren'an1
KeywordMetal-free Porous Graphitic Carbon Metal-organic Framework Glycan Profiling Enrichment Size Exclusion
Source PublicationACS APPLIED MATERIALS & INTERFACES
2018-04-11
ISSN1944-8244
DOI10.1021/acsami.8b02423
Volume10Issue:14Pages:11896-11906
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordOXYGEN REDUCTION REACTION ; MALDI-TOF-MS ; MASS-SPECTROMETRY ; SELECTIVE ENRICHMENT ; LASER DESORPTION/IONIZATION ; LIQUID-CHROMATOGRAPHY ; GRAPHENE NANORIBBONS ; NANOPOROUS CARBONS ; FACILE PREPARATION ; HIGH-THROUGHPUT
AbstractIn this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material. The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m(2)/g. After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism. Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 mu g/mu L, and the detection limit of glycans could be down to 1 ng/mu L, with 21 N-linked glycans identified. When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified. From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon. In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
Language英语
WOS IDWOS:000430156000058
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169079
Collection中国科学院大连化学物理研究所
Corresponding AuthorWu, Ren'an
Affiliation1.Chinese Acad Sci, Lab High Resolut Mass Spectrometry Technol, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Xin,Xu, Guiju,Peng, Jiaxi,et al. Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(14):11896-11906.
APA Li, Xin.,Xu, Guiju.,Peng, Jiaxi.,Liu, Shengju.,Zhang, Hongyan.,...&Wu, Ren'an.(2018).Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.ACS APPLIED MATERIALS & INTERFACES,10(14),11896-11906.
MLA Li, Xin,et al."Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans".ACS APPLIED MATERIALS & INTERFACES 10.14(2018):11896-11906.
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