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Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation
Wu, Ci1,2,3; Liang, Yu1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
KeywordHybrid Monolith Mesoporosity Ethane-bridged Organosilane Peptide Separation Proteomics
Source PublicationANALYTICA CHIMICA ACTA
2018-08-17
ISSN0003-2670
DOI10.1016/j.aca.2018.02.033
Volume1019Pages:128-134
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCAPILLARY LIQUID-CHROMATOGRAPHY ; ONE-POT PROCESS ; POROUS POLYMER MONOLITHS ; PEAK-CAPACITY ; SILICA MONOLITHS ; SMALL MOLECULES ; COLUMN ; LC ; ELECTROCHROMATOGRAPHY ; OPTIMIZATION
AbstractA novel kind of hybrid monolith with well-defined mesopore structure was prepared based on sol-gel condensation of 1, 2-bis(trimethoxysilyl) ethane (BTME) and tetramethoxysilane. Compared with terminal organosiloxanes used for preparation of conventional hybrid monoliths, BTME, as an ethanebridged alkoxysilane precursor, could not only maintain the uniformity of pore size distribution, but also improve the chemical stability of the monolith via Si-C bonds in the framework. Owing to the controllable mesoporous structure and good stability, the monolithic column was used for the separation of peptides with half peak width less than 6 s and the run-to-run and column-to-column relative standard deviations (RSD) for the retention time of five standard peptides less than 2.5%, showing narrow peak width and good reproducibility. Moreover, the separation performance could be well maintained even after washed by the mobile phase with pH 11.0 at 50 degrees C. Furthermore, 100 cm-length monolithic column was prepared and successfully used for nanoRPLC-ESI-MS/MS analysis of HeLa cell lysate digests, and 5670 proteins corresponding to 37574 peptides were identified from 750 ng of the sample, showing great promising of "single-shot" large-scale in-depth proteomic research. (c) 2018 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000429177600013
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169089
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Inspect & Quarantine Tech Ctr Dalian Entry Exit I, Dalian 116100, Peoples R China
Recommended Citation
GB/T 7714
Wu, Ci,Liang, Yu,Liang, Zhen,et al. Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation[J]. ANALYTICA CHIMICA ACTA,2018,1019:128-134.
APA Wu, Ci,Liang, Yu,Liang, Zhen,Zhang, Lihua,&Zhang, Yukui.(2018).Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation.ANALYTICA CHIMICA ACTA,1019,128-134.
MLA Wu, Ci,et al."Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation".ANALYTICA CHIMICA ACTA 1019(2018):128-134.
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